A click-reactive fluorescent dye
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3-azido-7-hydroxy Coumarin

Item No. 10596

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3-azido-7-hydroxy-2H-1-benzopyran-2-one
CAS Number
817638-68-9
Synonyms
  • 3-azido-7-Hydroxycoumarin
  • AHC
  • Azidohydroxycoumarin
Molecular Formula
C9H5N3O3
Formula Weight
203.2
Purity
≥95%
Emission
477 nm
Excitation
404 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Acetonitrile: SolubleDMSO: SolubleMethanol: Soluble
SMILES
O=C1C(N=[N+]=[N-])=CC2=CC=C(O)C=C2O1
InChi Code
InChI=1S/C9H5N3O3/c10-12-11-7-3-5-1-2-6(13)4-8(5)15-9(7)14/h1-4,13H
InChi Key
KKJNQKVCZCNJDI-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 mg

    $109

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    100 mg

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    250 mg

    $409

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    500 mg

    $763

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    Product Description

    3-azido-7-hydroxy Coumarin is a click-reactive fluorescent dye.1,2 Upon copper(I)-catalyzed azide/alkyne cycloaddition (CuAAC) in a biological system, a fluorescent product is produced that displays excitation/emission maxima of 404 and 477 nM, respectively. 3-azido-7-hydroxy Coumarin has been used for spatially restricted metabolite labeling and the detection of foodborne pathogens.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Mishra, P.K., Sharma, N., Kim, H., et al. Gen-Click: Genetically encodable click reactions for spatially restricted metabolite labeling. ACS Cent. Sci. 9(8), 1650-1657 (2023).

    2. You, H., Wang, M., Wang, S., et al. Ultrasensitive and specific phage@DNAzyme probe-triggered fluorescent click chemistry for on-site detection of foodborne pathogens using a smartphone. Anal. Chem. 95(30), 11211-11218 (2023).