For immunochemical detection of DARPP-32 (Phospho-Thr34)
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DARPP-32 (Phospho-Thr34) Polyclonal Antibody

Item No. 10603

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr34 site of rat DARPP-32
MW
~32 kDa
Formulation (Request formulation change)
100 µl of Affinity-purified rabbit polyclonal antibody
Storage Buffer
10 mM HEPES, pH 7.5, with 150 mM sodium chloride, 100 µg BSA per ml, and 50% glycerol
Host
Rabbit
Applications
WB
Cross Reactivity
(+) DARPP-32 (phospho-Thr34)(-) Unphosphorylated DARPP-32
Species Reactivity
(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µl

    $476

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    Product Description

    Dopamine and cAMP-regulated phosphoprotein of molecular weight 32 kDa (DARPP-32) is a member of the protein phosphatase inhibitor 1 family and is encoded by the PPP1R1B gene in humans.1 It is primarily expressed in medium spiny neurons of dopamine-innervated brain regions and is localized to the cytoplasm.2 DARPP-32 contains multiple phosphorylation sites that allow it to function as a central regulator and integrator of signaling cascades induced by a variety of neurotransmitters, neuropeptides, and psychostimulatory agents. It also contains a protein phosphatase 1 (PP1) binding domain.3 DARPP-32, when phosphorylated at the threonine in position 34 (phospho-Thr34) by PKA, is a PP1 inhibitor.1 Phosphorylation of DARPP-32 at Thr34 is induced by GABA in rat striatal and substantia nigra slices.4 Mice expressing a point mutation of Thr34 (T34A) in DARPP-32, which abolishes its phosphorylation, have reduced cocaine-induced conditioned place preference compared to wild-type mice.5 DARPP-32 (phospho-Thr34) levels are decreased in postmortem-derived prefrontal cortex from individuals with schizophrenia.6 Cayman's DARPP-32 (Phospho-Thr34) Polyclonal Antibody can be used for Western blot (WB) applications. The antibody recognizes DARPP-32 (phospho-Thr34) at approximately 32 kDa from mouse and rat samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Meyer-Lindenberg, A., Straub, R.E., Lipska, B.K., et al. Genetic evidence implicating DARPP-32 in human frontostriatal structure, function, and cognition. J. Clin. Invest. 117(3), 672-682 (2007).

    2. Yger, M., and Girault, J.A. DARPP-32, jack of all trades… master of which? Front. Behav. Neurosci. 5, 56 (2011).

    3. Greengard, P., Allen, P.B., and Nairn, A.C. Beyond the dopamine receptor: The DARPP-32/protein phosphatase-1 cascade. Neuron. 23(3), 435-447 (1999).

    4. Snyder, G.L., Fisone, G., and Greengard, P. Phosphorylation of DARPP-32 is regulated by GABA in rat striatum and substantia nigra. J. Neurochem. 63(5), 1766-1771 (1994).

    5. Zachariou, V., Sgambato-Faure, V., Sasaki, T., et al. Phosphorylation of DARPP-32 at threonine-34 is required for cocaine action. Neuropsychopharmacology 31(3), 555-562 (2006).

    6. Wang, H., Farhan, M., Xu, J., et al. The involvement of DARPP-32 in the pathophysiology of schizophrenia. Oncotarget 8(32), 53791-53803 (2017).