A fluorescent probe for HDAC
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coumarin-SAHA

Item No. 10671

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N1-hydroxy-N8-(4-methyl-2-oxo-2H-chromen-7-yl)-octanediamide
CAS Number
1260635-77-5
Synonyms
  • coumarin-Suberoylanilide Hydroxamic Acid
Molecular Formula
C18H22N2O5
Formula Weight
346.4
Purity
≥98%
Emission
400 nm
Excitation
325 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMF:PBS (pH 7.2) (1:9): 0.1 mg/mlDMSO: 5 mg/ml
λmax
229, 328 nm
SMILES
O=C(CCCCCCC(NO)=O)NC1=CC2=C(C(C)=CC(O2)=O)C=C1
InChi Code
InChI=1S/C18H22N2O5/c1-12-10-18(23)25-15-11-13(8-9-14(12)15)19-16(21)6-4-2-3-5-7-17(22)20-24/h8-11,24H,2-7H2,1H3,(H,19,21)(H,20,22)
InChi Key
YSRCADVJNRJJAV-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $40

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    5 mg

    $98

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    10 mg

    $147

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    25 mg

    $274

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    Product Description

    coumarin-Suberoylanilide hydroxamic acid (c-SAHA) is a SAHA derivative where the anilino “cap” group is replaced by 7-amino-4-methylcoumarin to produce a fluorescent probe that competitively binds HDAC. 1 The fluorescence excitation and emission maxima of free c-SAHA is 325 and 400 nm, respectively and is quenched by 50% when bound to HDAC8.1 This probe can be used to determine binding affinities and dissociation off-rates of HDAC enzyme-inhibitor complexes and is well-suited for high-throughput screening.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Singh, R.K., Mandal, T., Balasubramanian, N., et al. Coumarin-suberoylanilide hydroxamic acid as a fluorescent probe for determining binding affinities and off-rates of histone deacetylase inhibitors. Anal. Biochem. 408(2), 309-315 (2011).