Pure human recombinant enzyme
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O6-methylguanine-DNA Methyltransferase (human, recombinant)

Item No. 11176

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • MGMT
Purity
≥50% estimated by SDS-PAGE
Source
Recombinant N-terminal His-tagged protein expressed in E. coli
Amino Acids
2-238 (full length)
MW
26.8 kDa
Formulation (Request formulation change)
50 mM Tris, pH 8.0, 150 mM sodium chloride, 10% glycerol
UniProt Accession №
B4DEE8
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $423

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    Product Description

    O6-methylguanine-DNA Methyltransferase (MGMT) is a DNA repair enzyme responsible for demethylating O6-methylguanine (O6-mG).1 The methylation of guanine at O6 to form O6-mG allows base-pairing to thymine rather than cytosine during DNA replication. MGMT prevents mutations by transferring the methyl group from O6-mG to MGMT cysteine 145, restoring guanine. This transfer results in a covalent bond between MGMT cysteine 145 and the methyl group, and so MGMT is a single-turnover, "suicide" enzyme. The assay used to test MGMT is the demethylation of a synthesized, double-stranded, DNA oligonucleotide.2 Demethylation removes an O6-methyl group to expose a restriction site for the restriction endonuclease PvuII.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Mitra, S. MGMT: A personal perspective. DNA Repair (Amst) 6(8), 1064-1070 (2007).

    2. Watts, G.S., Pieper, R.O., Costello, J.F., et al. Methylation of discrete regions of the O6-methylguanine DNA methyltransferase (MGMT) CpG island is associated with heterochromatinization of the MGMT transcription start site and silencing of the gene. Mol. Cell. Biol. 17(9), 5612-5619 (1997).