A fluorescent DNA dye
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7-Aminoactinomycin D

Item No. 11397

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-amino-actinomycin D
CAS Number
7240-37-1
Synonyms
  • 7-AAD
  • NSC 239759
Molecular Formula
C62H87N13O16
Formula Weight
1270.4
Purity
≥95%
Emission
650 nm
Excitation
488, 546, 578 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMF:PBS(pH 7.2)(1:1): 0.5 mg/mlDMSO: 5 mg/ml
λmax
240, 529 nm
SMILES
O=C1C(N)=C(C(NC(C(C)OC(C(N(C2=O)C)C(C)C)=O)C(NC(C(C)C)C(N(CCC3)C3C(N(C)C2)=O)=O)=O)=O)C4=NC5=C(C(NC(C(OC(C(N(C(CN6C)=O)C)C(C)C)=O)C)C(NC(C(C)C)C(N7CCCC7C6=O)=O)=O)=O)C=C(N)C(C)=C5OC4=C1C
InChi Code
InChI=1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-
InChi Key
YXHLJMWYDTXDHS-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $121

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    5 mg

    $477

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    Product Description

    7-Aminoactinomycin D (7-AAD) is a fluorescent DNA dye that is commonly used for the detection or exclusion of non-viable cells in flow cytometric analysis, as it is generally excluded by live cells.1,2 It displays excitation spectra of 488, 546, and 578 nm and an emission spectrum of 650 nm.3 As 7-AAD is detected in the far red range of the spectrum, it exhibits minimal spectral overlap with commonly used probes, therefore it can be used in conjunction with probes such as FITC.1,3 7-AAD has been used to evaluate apoptosis and cell-mediated cytotoxicity and to stain DNA in cells that have been fixed and permeabilized by a variety of methods.1,2,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schmid, I., Uittenbogaart, C.H., Keld, B., et al. A rapid method for measuring apoptosis and dual-color immunofluorescence by single laser flow cytometry. J. Immunol. Methods 170(2), 145-157 (1994).

    2. Coder, D.M. Assessment of cell viability. Curr. Protoc. Cytom. Suppl. 15, (1997).

    3. Stokke, T., Holte, H., and Steen, H.B. In vitro and in vivo activation of B-lymphocytes: A flow cytometric study of chromatin structure employing 7-aminoactinomycin D. Cancer Res. 48(23), 6708-6714 (1988).

    4. Lecoeur, H., Février, M., Garcia, S., et al. A novel flow cytometric assay for quantitation and multiparametric characterization of cell-mediated cytotoxicity. J. Immunol. Methods 253(1-2), 177-187 (2001).