AA: 2-185 (C-terminal truncation) • Tag: N-terminal GST • MW: 48.2 kDa
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ORC1 BAH domain (human, recombinant)

Item No. 11919

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • ORC1L
  • Origin Recognition Complex Subunit 1
  • PARC1
  • Replication Control Protein 1
Purity
≥80%
Source
Recombinant N-terminal GST-tagged protein expressed in E. coli
Amino Acids
2-185 (C-terminal truncation)
MW
48.2 kDa
Formulation (Request formulation change)
20 mM Tris, pH 8.0, with 50 mM sodium chloride and 20% glycerol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 mg

    $659

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    Product Description

    ORC1, a component of the Origin Recognition Complex, is necessary for the initiation of DNA replication. It is bound in complex with ORC2, ORC3, ORC4, ORC5, and ORC6. The BromoAdjacent Homology (BAH) domain of ORC1 recognizes histone 4 dimethylated at lysine 20, which is enriched at replication origins (ORI).1 Chromatin immunoprecipitation and parallel sequencing of ORC1 DNA binding sites has been used to identify the ORI sites in HeLa cells.2 In addition to BAH mediated nucleosome recognition, ORC1 contains ATPase activity which is required for loading the minichromosome maintenance helicase complex.3 ORC1 levels oscillate during the cell cycle, decreasing during S phase following formation of pre-replication complexes.4 Mutations in ORC1 have been shown to cause Meier-Gorlin syndrome, a form of primordial dwarfism.5 This protein product contains the BAH domain region of ORC1.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kuo, A.J., Song, J., Cheung, P., et al. The BAH domain of ORC1 links H4K20me2 to DNA replication licensing and Meier-Gorlin syndrome. Nature 484, 115-119 (2012).

    2. Dellino, G.I., Cittaro, D., Piccioni, R., et al. Genome-wide mapping of human DNA-replication origins: Levels of transcription at ORC1 sites regulate origin selection and replication timing. Genome Res. 23(1), 1-11 (2013).

    3. Fernández-Cid, A., Riera, A., Tognetti, S., et al. An ORC/Cdc6/MCM2-7 complex is formed in a multistep reaction to serve as a platform for MCM double-hexamer assembly. Mol. Cell 50(4), 577-588 (2013).

    4. Tatsumi, Y., Ohta, S., Kimura, H., et al. The ORC1 cycle in human cells: I. Cell cycle-regulated oscillation of human ORC1. The Journal of Biological Chemisty 278(42), 41528-41534 (2003).

    5. Klingseisen, A., and Jackson, A.P. Mechanisms and pathways of growth failure in primordial dwarfism. Genes Dev. 25(19), 2011-2024 (2011).