A fluorescent dye to monitor superoxide production
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Dihydroethidium

Item No. 12013

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-ethyl-5,6-dihydro-6-phenyl-3,8-phenanthridinediamine
CAS Number
104821-25-2
Synonyms
  • Hydroethidine
  • PD-MY 003
Molecular Formula
C21H21N3
Formula Weight
315.4
Purity
≥98%
Emission
590 nm
Excitation
490 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 0.5 mg/mlDMSO: 10 mg/mlDMSO:PBS(pH7.2) (1:1): 0.5 mg/mlEthanol: 0.25 mg/ml
λmax
224, 271, 354 nm
SMILES
NC1=CC2=C(C3=C(C=C(N)C=C3)N(CC)C2C4=CC=CC=C4)C=C1
InChi Code
InChI=1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
InChi Key
XYJODUBPWNZLML-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $53

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    10 mg

    $101

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    25 mg

    $225

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    Product Description

    Dihydroethidium is a cell-permeable blue fluorescent dye that upon entering cells interacts with superoxide to form oxyethidium, which intercalates with nucleic acids and emits a red fluorescence detectable qualitatively by fluorescent microscopy or quantitatively by HPLC.1 It displays excitation/emission spectra of 490/590 nm.2 This selective and sensitive probe has been used to detect reactive oxygen species during the phagocytic respiratory burst and for the detection of intracellular superoxide in cultured cells.3,4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cai, H., Dikalov, S., Griendling, K.K., et al. Detection of reactive oxygen species and nitric oxide in vascular cells and tissues: Comparison of sensitivity and specificity. Methods Mol. Med. 139, 293-311 (2007).

    2. Laurindo, F.R.M., Fernandes, D.C., and Santos, C.X.C. Assessment of superoxide production and NADPH oxidase activity by HPLC analysis of dihydroethidium oxidation products. Methods Enzymol. 441, 237-260 (2008).

    3. Fink, B., Laude, K., McCann, L., et al. Detection of intracellular superoxide formation in endothelial cells and intact tissues using dihydroethidium and an HPLC-based assay. Am. J. Physiol. Cell Physiol. 287, C895-C902 (2004).

    4. Robinson, K.M., Janes, M.S., Pehar, M., et al. Selective fluorescent imaging of superoxide in vivo using ethidium-based probes. Proc. Natl. Acad. Sci. USA 103(41), 15038-15043 (2006).

    5. Salih, H.R., Husfeld, L., and Adam, D. Simultaneous cytofluorometric measurement of phagocytosis, burst production and killing of human phagocytes using Candida albicans and Staphylococcus aureus as target organisms. Clin. Microbiol. Infect. 6(5), 251-258 (2000).