Fluorescent probe for thiol bioimaging
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ThioFluor 623

Item No. 13083

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[4-[(1E)-2-[4-cyano-5-(dicyanomethylene)-2,5-dihydro-2,2-dimethyl-3-furanyl]ethenyl]phenyl]-N-[2-[2-(2-methoxyethoxy)ethoxy]ethyl]2,4-dinitro-benzenesulfonamide
CAS Number
1004324-99-5
Synonyms
  • Fluorescent Thiol Probe
Molecular Formula
C31H30N6O10S
Formula Weight
678.7
Purity
≥98%
Emission
623 nm
Excitation
563 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 20 mg/ml
SMILES
COCCOCCOCCN(c1ccc(cc1)\C=C/C1=C(CN)C(=C(CN)CN)OC1(C)C)S(=O)(=O)c1ccc(cc1[N](=O)O)[N](=O)O
InChi Code
InChI=1S/C31H30N6O10S/c1-31(2)27(26(21-34)30(47-31)23(19-32)20-33)10-6-22-4-7-24(8-5-22)35(12-13-45-16-17-46-15-14-44-3)48(42,43)29-11-9-25(36(38)39)18-28(29)37(40)41/h4-11,18H,12-17H2,1-3H3/b10-6+
InChi Key
CDPMXQUCTGVMFA-UXBLZVDNSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 mg

    $197

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    10 mg

    $376

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    50 mg

    $985

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    Product Description

    The rapid, selective, and sensitive sensing of thiols is important in diverse areas of research. This Thiofluor 623 responds upon exposure to thiols with an increase in fluorescence intensity of up to 120-fold.1 The response is selective for thiols and occurs in aqueous media.1 In the absence of thiols, the probe is essentially non-fluorescent; thiols cause cleavage of the probe, generating a fluorophore with an absorption maximum of 563 nm and emission at 623 nm.1 The fluorescence quantum yield of the cleaved product, generated in response to thiols, increases in more viscous media, suggesting ideal performance in biological systems and applicability to single-molecule or 2-photon sensing schemes. The Thiofluor 623 is cell-permeable and reacts selectively with intracellular thiols.1 The pseudo-first order rate constant (kobs) depends on substrate (e.g., 2.1 x 10−3 s−1 for cysteine, 2.0 x 10−5 s−1 for human serum albumin).1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bouffard, J., Kim, Y., Swager, T.M., et al. A highly selective fluorescent probe for thiol bioimaging. Org. Lett. 10(1), 37-40 (2008).