A thiol-reactive fluorescent probe
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

ThioGlo1

Item No. 13235

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
10-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)-9-methoxy-3-oxo-H-naphtho[2,1-b]pyran-2-carboxylic acid, methyl ester
CAS Number
137350-66-4
Synonyms
  • ThioGlo-1
Molecular Formula
C20H13NO7
Formula Weight
379.3
Purity
≥95%
Emission
513 nm
Excitation
384 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/mlDMSO:PBS (pH 7.2) (1:1): 0.50 mg/ml
λmax
216, 238, 244 nm
SMILES
COC1=CC=C(C=CC(O2)=C3C=C(C(OC)=O)C2=O)C3=C1N4C(C=CC4=O)=O
InChi Code
InChI=1S/C20H13NO7/c1-26-14-6-4-10-3-5-13-11(9-12(19(24)27-2)20(25)28-13)17(10)18(14)21-15(22)7-8-16(21)23/h3-9H,1-2H3
InChi Key
NLQBAJWAMARDBZ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    10 mg

    $81

    Add

    50 mg

    $279

    Add

    100 mg

    $475

    Add

    250 mg

    $789

    Add
    Product Description

    ThioGlo1 is a thiol-reactive fluorescent probe.1,2,3 Upon reaction with a thiol group, a fluorescent adduct is formed that displays excitation/emission maxima of 384/513 nm, respectively.2,3 It also reacts with sulfite to form a fluorescent adduct with similar spectral characteristics, and interference from sulfite during thiol determination must be accounted for using secondary methods. ThioGlo1 has been used to monitor the oxidative stability of beer.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yang, J.-R., and Langmuir, M.E. Synthesis and properties of a maleimide fluorescent thiol reagent derived from a naphthopyranone. J. Heterocycl. Chem. 28(5), 1177-1180 (1991).

    2. Hoff, S., Larsen, F.S., Anderson, M.L., et al. Quantification of protein thiols using ThioGlo 1 fluorescent derivatives and HPLC separation. Analyst 138(7), 2096-2103 (2013).

    3. Lund, M.N., and Andersen, M.L. Detection of thiol groups in beer and their correlation with oxidative stability. J. Am. Soc. Brew. Chem. 69(3), 163-169 (2011).