For immunochemical detection of IκBα (phospho-Ser32/36)
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IκBα (Phospho-Ser32/36) Monoclonal Antibody (Clone 39A1413)

Item No. 13923

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • I-kappa-B-alpha
  • IkappaBalpha
  • IKBA
  • MAD-3
  • MAD3
  • Major Histocompatibility Complex Enhancer-binding Protein
  • NF-kappa-B Inhibitor alpha
  • NF-κB Inhibitor α
  • NFKBIA
Immunogen
A synthetic peptide containing phosphorylated serines at amino acid residues 32 and 36 of human IκBα
Source
32-36
Clone Designation
39A1413
Formulation (Request formulation change)
100 µg of protein G-purified IgG in 200 µl PBS containing 0.05% BSA and 0.05% gelatin and 0.05% sodium azide
Host
Mouse
Isotype
IgG1κ
Applications
IP, WB
Cross Reactivity
(+) IκBα (phospho-Ser32/36)
Species Reactivity
(+) Human IκBα(+) Bovine IκBα(+) Canine IκBα(+) Mouse IκBα(+) Porcine IκBα(+) Rat IκBα
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    IκBα is an inhibitor of NF-κB and a member of the IκB family of proteins.1,2,3 It is composed of an N-terminal signal-receiving domain, which contains several post-translational modification sites that regulate its activity, an ankyrin-repeat domain, which contains six ankyrin repeats that mediate protein-protein interactions with NF-κB dimers, a proline-glutamic acid-serine-threonine (PEST) domain, and two nuclear export sequences.2 IκBα is ubiquitously expressed and shuttles between the cytoplasm and nucleus.1,2,3 IκBα is phosphorylated by the IκB kinase (IKK) complex, which is activated via downstream signaling events stimulated by TNF-α, other cytokines, viruses, or bacteria, and its phosphorylation leads to its ubiquitination and degradation by the proteasome. The degradation of IκBα results in unmasking of the nuclear localization signal of NF-κB dimers, which subsequently translocate to the nucleus and activate target genes. Mutations in NFKBIA, the gene encoding IκBα, are associated with anhidrotic ectodermal dysplasia with immunodeficiency.4 Cayman’s IκBα (Phospho-Ser32/36) Monoclonal Antibody (Clone 39A1413) can be used for immunoprecipitation (IP) and Western blot (WB) applications. The antibody recognizes IκBα (phospho-Ser32/36) from human, mouse, rat, porcine, bovine, and dog samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Majdalawieh, A., and Ro, H.-S. Regulation of IκBα function and NF-κB signaling: AEBP1 is a novel proinflammatory mediator in macrophages. Mediators Inflamm. 823821 (2010).

    2. Wang, X., Peng, H., Huang, Y., et al. Post-translational modifications of IκBα: The state of the art. Front. Cell Dev. Biol. 8, 574706 (2020).

    3. Verma, I.M., Stevenson, J.K., Schwarz, E.M., et al. Rel/NF-κB/IκB family: Intimate tales of association and dissociation. Genes Dev. 9(22), 2723-2735 (1995).

    4. Boisson, B., Puel, A., Picard, C., et al. Human IκBα gain of function: A severe and syndromic immunodeficiency. J. Clin. Immunol. 37(5), 397-412 (2017).