A bacterial metabolite with diverse biological activities
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Bafilomycin B1

Item No. 14005

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-[(2-hydroxy-5-oxo-1-cyclopenten-1-yl)amino]-4-oxo-2E-butenoic acid, (2R,4R,5S,6R)-tetrahydro-2-hydroxy-2-[(1S,2R,3S)-2-hydroxy-3-[(2R,3S,4E,6E,9S,10S,11R,12E,14Z)-10-hydroxy-3,15-dimethoxy-7,9,11,13-tetramethyl-16-oxooxacyclohexadeca-4,6,12,14-tetraen-2-yl]-1-methylbutyl]-5-methyl-6-(1-methylethyl)-2H-pyran-4-yl ester
CAS Number
88899-56-3
Synonyms
  • Setamycin
Molecular Formula
C44H65NO13
Formula Weight
816.0
Purity
≥97%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: SolubleMethanol: Unstable
SMILES
C[C@H]([C@@](C[C@H]1OC(/C=C/C(NC(C(CC2)=O)=C2O)=O)=O)(O[C@H](C(C)C)[C@H]1C)O)[C@H](O)[C@@H]([C@](OC(/C(OC)=C/C(C)=C/[C@@H](C)[C@@H](O)[C@@H](C)C3)=O)([H])[C@H](/C=C/C=C3\C)OC)C
InChi Code
InChI=1S/C44H65NO13/c1-23(2)41-28(7)35(56-37(49)18-17-36(48)45-38-31(46)15-16-32(38)47)22-44(53,58-41)30(9)40(51)29(8)42-33(54-10)14-12-13-24(3)19-26(5)39(50)27(6)20-25(4)21-34(55-11)43(52)57-42/h12-14,17-18,20-21,23,26-30,33,35,39-42,46,50-51,53H,15
InChi Key
KFUFLYSBMNNJTF-ANDWMEETSA-N
Origin
Bacterium/Streptomyces hygroscopicus
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    100 µg

    $46

    Add

    1 mg

    $157

    Add

    5 mg

    $556

    Add
    Cayman Chemical
    Neutrophil Biology Wall Poster

    Explore how neutrophils shape the immune response in health and disease. This poster highlights neutrophil pathogen defense mechanisms, including phagocytosis, degranulation, and NETosis, as well as neutrophil roles in inflammation and NET-associated pathologies.

    DOWNLOAD NOW
    Product Description

    Bafilomycin B1 is a bacterial metabolite that has been found in Streptomyces and has diverse biological activities.1,2,3,4,5,6 It inhibits the activity of rat liver lysosomal vacuolar H+-ATPase (V-ATPase) in a concentration-dependent manner.4 Bafilomycin B1 (10-1,000 nM) induces autophagosome accumulation in MCF-7 cells.5 It inhibits chloroquine-induced apoptosis in primary cerebellar granule neurons (CGNs) when used at a concentration of 1 nM.3 Bafilomycin B1 reduces viral genome copy numbers in the culture supernatant of Vero E6 cells infected with H1N1 influenza A.6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bowman, E.J., Siebers, A., and Altendorf, K. Bafilomycins: A class of inhibitors of membrane ATPases from microorganisms, animal cells, and plant cells. Proc. Natl. Acad. Sci. USA 85(21), 7972-7976 (1988).

    2. Yamamoto, A., Tagawa, Y., Yoshimori, T., et al. Bafilomycin A1 prevents maturation of autophagic vacuoles by inhibiting fusion between autophagosomes and lysosomes in rat hepatoma cell line, H-4-II-E cells. Cell Struct. Funct. 23(1), 33-42 (1998).

    3. Shacka, J.J., Klocke, B.J., and Roth, K.A. Autophagy, bafilomycin and cell death: The "A-B-Cs" of plecomacrolide-induced neuroprotection. Autophagy 2(3), 228-230 (2006).

    4. Papini, E., de Bernard, M., Bugnoli, M., et al. Cell vacuolization induced by Helicobacter pylori: Inhibition by bafilomycins A1, B1, C1 and D. FEMS Microbiol. Lett. 113(2), 155-159 (1993).

    5. Carr, G., Williams, D.E., Díaz-Marrero, A.R., et al. Bafilomycins produced in culture by Streptomyces spp. isolated from marine habitats are potent inhibitors of autophagy. J. Nat. Prod. 73(3), 422-427 (2010).

    6. Xie, X., Lu, S., Pan, X., et al. Antiviral bafilomycins from a feces-inhabiting Streptomyces sp. J. Nat. Prod. 84(2), 537-543 (2021).