A cell-permeable, reversible inhibitor of DNA polymerases
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

(+)-Aphidicolin

Item No. 14007

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(3R,4R,4aR,6aS,8R,9R,11aS,11bS)-tetradecahydro-3,9-dihydroxy-4,11b-dimethyl-8,11a-methano-11aH-cyclohepta[a]naphthalene-4,9-dimethanol
CAS Number
38966-21-1
Synonyms
  • ICI 69653
  • NSC 234714
Molecular Formula
C20H34O4
Formula Weight
338.5
Purity
≥98%
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: 50 mg/mlMethanol: 10 mg/ml
SMILES
O[C@]1(CO)CC[C@]23[C@@](C[C@@H]1C3)([H])CC[C@@]4([H])[C@@](CO)(C)[C@H](O)CC[C@@]42C
InChi Code
InChI=1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1
InChi Key
NOFOAYPPHIUXJR-APNQCZIXSA-N
Origin
Fungus/Phoma sp. BS 7210
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $105

    Add

    5 mg

    $340

    Add

    25 mg

    $1,176

    Add
    Lipid Resource Center
    Discover Products & Resources for Lipid Research
    • High-purity lipid standards
    • Lipid roles in biology
    • Lipids in health & disease
    • Lipids for pharmaceutical development
    • Protocols, advice, & resources
    EXPLORE NOW
    Product Description

    (+)-Aphidicolin is a natural tetracyclic diterpene first isolated from the fungus C. aphidicola and shown to have antiviral activity against herpes simplex.1 In eukaryotic cells, it is a cell-permeable, reversible inhibitor of DNA replication, specifically blocking the activity of DNA polymerases α, δ, and ε when used at low micromolar levels.2,3,4 Aphidicolin can be used, at 3 μM, to arrest cells in G1/S phase or to increase gene amplification frequency.5,6

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bucknall, R.A., Moores, H., Simms, R., et al. Antiviral effects of aphidicolin, a new antibiotic produced by Cephalosporium aphidicola. Antimicrob. Agents Chemother. 4(3), 294-298 (1973).

    2. Oguro, M., Suzuki-Hori, C., Nagano, H., et al. The mode of inhibitory action by aphidicolin on eukaryotic DNA polymerase α. Eur. J. Biochem. 97(2), 603-607 (1979).

    3. Krokan, H., Wist, E., and Krokan, R.H. Aphidicolin inhibits DNA synthesis by DNA polymerase α and isolated nuclei by a similar mechanism. Nucleic Acids Res. 9(18), 4709-4719 (1981).

    4. Cheng, C.H., and Kuchta, R.D. DNA polymerase ε: Aphidicolin inhibition and the relationship between polymerase and exonuclease activity. Biochemistry 32(33), 8568-8574 (1993).

    5. Kota, K.P., Benko, J.G., Mudhasani, R., et al. High content image based analysis identifies cell cycle inhibitors as regulators of Ebola virus infection. Viruses 4(10), 1865-1877 (2012).

    6. Yin, D.X., and Schimke, R.T. Inhibition of apoptosis by overexpressing Bcl-2 enhances gene amplification by a mechanism independent of aphidicolin pretreatment. Proc. Natl. Acad. Sci. USA 93(8), 3394-3398 (1996).