For immunochemical detection of GPR31
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GPR31 (N-Term) Polyclonal Antibody

Item No. 14033

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 12-(S)-hydroxy-5,8,10,14-Eicosatetraenoic Acid Receptor
  • G Protein-Coupled Receptor 31
  • 12-HETER
  • 12(S)-HETE Receptor
Immunogen
A synthetic peptide from the N-terminal region of human GPR31
Formulation (Request formulation change)
500 µl peptide affinity-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
WB, ELISA, FC, and IF
Species Reactivity
(+) Human
UniProt Accession №
O00270
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (500 µl)

    $374

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    Cayman Chemical
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    Product Description

    GPR31, also known as the 12(S)-HETE receptor (12-HETER), is a G protein-coupled receptor that is coupled to the Gi signal transduction pathway and activated by 12(S)-HETE (Item No. 34570), a metabolite of arachidonic acid (Item Nos. 90010 | 90010.1 | 10006607).1,2 It is expressed by platelets and localizes to the plasma membrane and endoplasmic reticulum.1,3 siRNA knockdown of GPR31 inhibits 12(S)-HETE-induced tumor cell invasion in vitro, and Gpr31 knockdown in murine Hepa1-6 cells reduces tumorigenesis of those cells in a mouse model of hepatocellular carcinoma recurrence after hepatic ischemia-reperfusion injury.2,4 It is overexpressed in prostate cancer tumors and positively correlated with tumor grade.5 Cayman's GPR31 (N-Term) Polyclonal Antibody can be used for Western blot (WB), ELISA, flow cytometry (FC), and immunofluorescence (IF) applications. The antibody recognizes the N-terminal region of GPR31 from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Van Doren, L., Nguyen, N., Garzia, C., et al. Lipid receptor GPR31 (G-protein-coupled receptor 31) regulates platelet reactivity and thrombosis without affecting hemostasis. Arterioscler. Thromb. Vasc. Biol. 41(1), e33-e45 (2021).

    2. Guo, Y., Zhang, W., Giroux, C., et al. Identification of the orphan G protein-couple receptor GPR31 as a receptor for 12(S)-hydroxyeicosatetraenoic acid. The Journal of Biological Chemisty 286(39), 33832-33840 (2011).

    3. Fehrenbacher, N., and Philips, M.R. Targeting RAS - will GPR31 deliver us a new path forward? Mol. Cell. Oncol. 4(6), e1359228 (2017).

    4. Yang, F., Zhang, Y., Ren, H., et al. Ischemia reperfusion injury promotes recurrence of hepatocellular carcinoma in fatty liver via ALOX12-12HETE-GPR31 signaling axis. J. Exp. Clin. Cancer Res. 38(1), 489 (2019).

    5. Honn, K.V., Guo, Y., Cai, Y., et al. 12-HETER1/GPR31, a high-affinity 12(S)-hydroxyeicosatetraenoic acid receptor, is significantly up-regulated in prostate cancer and plays a critical role in prostate cancer progression. The FASEB Journal 30(6), 2360-2369 (2016).