Active, pure human recombinant enzyme
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Product Categories

Product Type

Proteins
Enzymes
Dehydrogenases

Research Area

Cancer

Application

Enzyme Activity
Western Blot

IDH1 (human recombinant)

Item No. 14131

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Isocitrate Dehydrogenase (NADP) Cytoplasmic
Purity
≥90% estimated by SDS-PAGE
Source
recombinant C-terminal His-tagged protein expressed in E. coli
MW
47.9 kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.5, containing 200 mM sodium chloride, 5 mM β-mercaptoethanol, 50 mM calcium chloride, and 20% glycerol
UniProt Accession №
O75874
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $602

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    Product Description

    Isocitrate dehydrogenases (IDHs) are NAD+ and NADP+-dependent enzymes that catalyze the third step of the tricarboxylic acid cycle. IDHs catalyze oxidative decarboxylation of isocitrate producing α-ketoglutarate and carbon dioxide. IDH1 (cytosolic) and IDH2 (mitochondrial) are NADP+-dependent enzymes that catalyze reversible reactions. The IDH3 isoform, a NAD+-dependent multisubunit enzyme, is irreversible and allosterically regulated by a variety of positive (calcium, ADP, and citrate) and negative (ATP, NADH, and NADPH) effectors.1 IDH1 and IDH2 are mutated in >70% of lower grade gliomas.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Raimundo, N., Baysal, B.E., and Shadel, G.S. Revisiting the TCA cycle: Signaling to tumor formation. Trends Mol. Med. 17(11), 641-649 (2011).

    2. Turcan, S., Rohle, D., Goenka, A., et al. IDH1 mutation is sufficient to establish the glioma hypermethylator phenotype. Nature 483(7390), 479-483 (2012).