Active • Host: E. coli • AA: 2-380 (full length) • Tag: C-terminal His • MW: 43.8 kDa
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

FEN1 (human, recombinant)

Item No. 14199

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Flap Endonuclease 1
  • hFEN1
Purity
≥85% estimated by SDS-PAGE
Source
Active recombinant C-terminal His-tagged protein expressed in E. coli
Amino Acids
2-380 (full length)
MW
43.8 kDa
Formulation (Request formulation change)
50 mM Tris, pH 8.0, with 1 mM DTT and 10% glycerol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    100 µg

    $436

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    Flap endonuclease 1 (FEN1), a RAD2 Class II nuclease, plays a crucial role in DNA repair, replication, and genomic stability.1 FEN1 removes the 5'-unannealed flap during Okazaki fragment processing in lagging strand DNA synthesis.2 Removal of the DNA flap is also critical in long-patch base excision repair and regulation of recombination.3 In yeast, FEN1 mutants display sensitivity to UV irradiation, deficient chromosome segregation, and cell cycle arrest in S phase.4 Furthermore, haploinsufficiency of FEN1 in mice leads to rapid tumor progression.4 FEN1 is overexpressed in many forms of cancer, including lung, gastric, prostate, pancreatic, brain, and breast.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lee, B.I., and Wilson, D.M., III The RAD2 domain of human exonuclease 1 exhibits 5' to 3' exonuclease and flap structure-specific endonuclease activities. The Journal of Biological Chemisty 274(53), 37763-37769 (1999).

    2. Jagannathan, I., Pepenella, S., and Hayes, J.J. Activity of FEN1 endonuclease on nucleosome substrates is dependent upon DNA sequence but not flap orientation. The Journal of Biological Chemisty 286(20), 17521-17529 (2011).

    3. Dorjsuren, D., Kim, D., Maloney, D.J., et al. Complementary non-radioactive assays for investigation of human flap endonuclease 1 activity. Nucleic Acids Res. 39(2), (2011).

    4. Sakurai, S., Kitano, K., Yamaguchi, H., et al. Structural basis for recruitment of human flap endonuclease 1 to PCNA. EMBO J. 24(4), 683-693 (2005).