A fluorescent DNA probe
Related Products
Alternative(s)
40796DAPI (hydrochloride) (solution)
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Fluorescence

DAPI (hydrochloride)

Item No. 14285

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2-[4-(aminoiminomethyl)phenyl]-1H-indole-6-carboximidamide, dihydrochloride
CAS Number
28718-90-3
Synonyms
  • 4',6-Diamidino-2-phenylindole
  • FxCycle Violet
Molecular Formula
C16H15N5 • 2HCl
Formula Weight
350.3
Emission
461 nm
Excitation
358 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 0.2 mg/mlDMSO: 3 mg/mlEthanol: 0.2 mg/ml
λmax
228, 264, 348 nm
SMILES
NC(C(C=C1)=CC=C1C2=CC3=CC=C(C(N)=N)C=C3N2)=N.Cl.Cl
InChi Code
InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChi Key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 mg

    $61

    Add

    10 mg

    $109

    Add

    25 mg

    $242

    Add
    Product Description

    DAPI is a fluorescent probe which is commonly used to stain DNA and chromosomes for fluorescent microscopy and flow cytometry applications.1,2 It forms a fluorescent complex by attaching in the minor groove of A-T rich sequences of DNA.1 DAPI is often used as a counterstain, as its ultraviolet (max 358 nm) excitation and blue (max 461 nm) emission wavelengths separate it nicely from many popular primary fluorophores. It can be used on either fixed or live cells, although its low permeability in live cells demands that higher concentrations be used.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kapuscinski, J. DAPI: A DNA-specific fluorescent probe. Biotech. Histochem. 70(5), 220-233 (1995).

    2. Zink, D., Sadoni, N., and Stelzer, E. Visualizing chromatin and chromosomes in living cells. Methods 29(1), 42-50 (2003).