A fluorescent probe used to identify dead cells
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Propidium Iodide

Item No. 14289

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,8-diamino-5-[3-(diethylmethylammonio)propyl]-6-phenyl-phenanthridinium, diiodide
CAS Number
25535-16-4
Synonyms
  • PI
Molecular Formula
C27H34N4 • 2I
Formula Weight
668.4
Purity
≥90%
Emission
617 nm
Excitation
488-535 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 3.3 mg/mlDMSO: 2.5 mg/mlEthanol: 0.2 mg/mlPBS (pH 7.2): 2 mg/ml
λmax
219, 298, 546 nm
SMILES
CC[N+](CC)(C)CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4.[I-].[I-]
InChi Code
InChI=1S/C27H33N4.2HI/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20;;/h6-8,10-15,18-19,29H,4-5,9,16-17,28H2,1-3H3;2*1H/q+1;;/p-1
InChi Key
XJMOSONTPMZWPB-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 mg

    $38

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    25 mg

    $66

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    50 mg

    $113

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    100 mg

    $187

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    Product Description

    Propidium iodide (PI) is membrane impermeant and generally excluded from viable cells. However, it can easily penetrate dead or damaged cells and as such is commonly used for identifying cell viability in a population or as a counterstain in multicolor fluorescent techniques.1 It binds to DNA and RNA by intercalating between the bases. PI is excited at 488-535 nm with an emission maximum of 617 nm. This probe is suitable for fluorescence microscopy and flow cytometry.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Coder, D.M. Assessment of cell viability. Curr. Protoc. Cytom. Suppl. 15, (1997).