An aminoglycoside antibiotic
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Labeled Version(s)
34443Hygromycin B-d4
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Hygromycin B (hydrate)

Item No. 14291

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
O-6-amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene- (1→2-3)-O-β-D-talopyranosyl- (1→5)-2-deoxy-N3-methyl-D-streptamine, hydrate
Molecular Formula
C20H37N3O13 • XH2O
Formula Weight
527.5
Purity
≥85%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 3.3 mg/mlPBS (pH 7.2): 3.3 mg/ml
SMILES
O[C@H]1[C@@H](CO)O[C@@H](O[C@@]2([H])[C@H](O)[C@@H](NC)C[C@@H](N)[C@@H]2O)[C@]3([H])[C@@]1([H])OC4(O[C@@]([C@@H](N)CO)([H])[C@H](O)[C@H](O)[C@H]4O)O3.O
InChi Code
InChI=1S/C20H37N3O13.H2O/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24;/h5-19,23-31H,2-4,21-22H2,1H3;1H2/t5-,6+,7+,8-,9+,10-,11+,12-,13+,14-,15-,16+,17+,18-,19+,20?;/m1./s1
InChi Key
FOVDUCWCXNHTHM-IGPOGLENSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    500 mg

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    Product Description

    Hygromycin B is an aminoglycoside antibiotic that has been found in S. hygroscopicus.1,2 It is active against E. coli (MIC = 150 µg/ml) and cytotoxic to both prokaryotic and eukaryotic cells via inhibition of protein synthesis.3,4,5 Hygromycin B has commonly been used in molecular and cell biology applications to select for transformed cells expressing the E. coli hygromycin resistance genes hyg or hph.4,5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Eustice, D.C., and Wilhelm, J.M. Mechanisms of action of aminoglycoside antibiotics in eucaryotic protein synthesis. Antimicrob. Agents Chemother. 26(1), 53-60 (1984).

    2. Cabañas, M.J., Vázquez, D., and Modolell, J. Dual interference of hygromycin B with ribosomal translocation and with aminoacyl-tRNA recognition. Eur. J. Biochem. 87(1), 21-27 (1978).

    3. McGaha, S.M., and Champney, W.S. Hygromycin B inhibition of protein synthesis and ribosome biogenesis in Escherichia coli. Antimicrob. Agents Chemother. 51(2), 591-596 (2006).

    4. Kaster, K.R., Burgett, S.G., Rao, R.N., et al. Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing. Nucleic Acids Res. 11(19), 6895-6911 (1983).

    5. Blochlinger, K., and Diggelmann, H. Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells. Mol. Cell Biol. 4(12), 2929-2931 (1984).

    Product Citations

    Zhao, J., Tang, Z., Selvaraju, M., et al. Cellular target deconvolution of small molecules using a selection-based genetic screening platform. ACS Cent. Sci. 8(10), 1424-1434 (2022).