An ENaC and NCX inhibitor
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Benzamil (hydrochloride)

Item No. 14313

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,5-diamino-6-chloro-N-[imino[(phenylmethyl)amino]methyl]-2-pyrazinecarboxamide, monohydrochloride
CAS Number
161804-20-2
Molecular Formula
C13H14ClN7O • HCl
Formula Weight
356.2
Purity
≥98%
Formulation
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 0.5 mg/mlDMSO: 20 mg/ml
λmax
210, 288, 363 nm
SMILES
ClC1=C(N)N=C(N)C(C(NC(NCC2=CC=CC=C2)=N)=O)=N1.Cl
InChi Code
InChI=1S/C13H14ClN7O.ClH/c14-9-11(16)20-10(15)8(19-9)12(22)21-13(17)18-6-7-4-2-1-3-5-7;/h1-5H,6H2,(H4,15,16,20)(H3,17,18,21,22);1H
InChi Key
ZNWMRWWNJBXNKJ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    5 mg

    $48

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    10 mg

    $94

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    Product Description

    Benzamil is a derivative of amiloride (Item No. 14409).1 Benzamil is an epithelial sodium channel (ENaC) blocker that inhibits sodium transport from (IC50 = 4 nM) and binds to (Kd = 5 nM) bovine kidney cortex membrane vesicles. It is also an inhibitor of the Na+/Ca2+ exchanger (NCX) that inhibits the cytosolic calcium response to extracellular sodium depletion in mouse podocytes (IC50 = ~100 nM).2 Benzamil inhibits transient receptor potential polycystin-L (TRPP3; IC50 = 1.1 μM), a member of the TRP superfamily of cation channels.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kleyman, T.R., Yulo, T., Ashbaugh, C., et al. Photoaffinity labeling of the epithelial sodium channel. The Journal of Biological Chemisty 261(6), 2839-2843 (1986).

    2. Fischer, K.-G., Jonas, N., Poschenrieder, F., et al. Characterization of a Na+-Ca2+ exchanger in podocytes. Nephrol. Dial. Transplant. 17(10), 1742-1750 (2002).

    3. Dai, X.Q., Ramji, A., Liu, Y., et al. Inhibition of TRPP3 channel by amiloride and analogs. Mol. Pharmacol. 72(6), 1576-1585 (2007).