A dye for staining or quantifying proteins
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Brilliant Blue G

Item No. 14320

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]-2-methylphenyl]methylene]-3-methyl-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-benzenemethanaminium, sodium salt
CAS Number
6104-58-1
Synonyms
  • Acid Blue 90
  • CBBG
  • Coomassie Brilliant Blue G-250
  • NSC 328382
Molecular Formula
C47H48N3O7S2 • Na
Formula Weight
854.0
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 0.5 mg/mlDMSO: 10 mg/mlEthanol: 0.5 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
202, 260, 309, 609 nm
SMILES
CC1=CC(N(CC)CC2=CC=CC(S(=O)([O-])=O)=C2)=CC=C1/C(C3=CC=C(NC4=CC=C(OCC)C=C4)C=C3)=C5C=C/C(C=C\5C)=[N+](CC6=CC=CC(S(=O)([O-])=O)=C6)/CC.[Na+]
InChi Code
InChI=1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30H,6-8,31-32H2,1-5H3,(H2,51,52,53
InChi Key
RWVGQQGBQSJDQV-UHFFFAOYSA-M
Origin
Synthetic
Shipping & Storage Information
Storage
Room temperature
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    Brilliant Blue G is a dye which is commonly used in laboratories to stain or quantify proteins. A variety of methods have been developed for using Brilliant Blue G to stain gels with ease and sensitivity.1,2,3,4 The Bradford assay is a standard, rapid dye-binding assay that uses Brilliant Blue G to quantify the amount of protein in a solution.5 This compound also acts as a selective inhibitor of the P2X purinoceptor channel P2X7 (IC50s = 10.1 and 265 nM for rat and human P2X7, respectively).6 In mice, it inhibits interleukin-1β expression and reduces neurological injury secondary to traumatic brain injury.7

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Diezel, W., Kopperschläger, G., and Hofmann, E. An improved procedure for protein staining in polyacrylamide gels with a new type of Coomassie brilliant blue. Anal. Biochem. 48(2), 617-620 (1972).

    2. Neuhoff, V., Stamm, R., and Eibl, H. Clear background and highly sensitive protein staining with Coomassie Blue dyes in polyacrylamide gels: A systematic analysis. Electrophoresis 6(9), 427-448 (1985).

    3. Candiano, G., Bruschi, M., Musante, L., et al. Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 25(9), 1327-1333 (2004).

    4. Pink, M., Verma, N., Rettenmeier, A.W., et al. CBB staining protocol with higher sensitivity and mass spectrometric compatibility. Electrophoresis 31(4), 593-598 (2010).

    5. Bradford, M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254 (1976).

    6. Jiang, L.H., Mackenzie, A.B., North, R.A., et al. Brilliant Blue G selectively blocks ATP-gated rat P2X7 receptors. Mol. Pharmacol. 58(1), 82-88 (2000).

    7. Kimbler, D.E., Shields, J., Yanasak, N., et al. Activation of P2X7 promotes cerebral edema and neurological injury after traumatic brain injury in mice. PLoS One 7(7), e41229 (2012).