A dye used as an oxidation-reduction indicator
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Resazurin (sodium salt)

Item No. 14322

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
7-hydroxy-3H-phenoxazin-3-one-10-oxide, monosodium salt
CAS Number
62758-13-8
Molecular Formula
C12H6NO4 • Na
Formula Weight
251.2
Purity
≥85%
Emission
580 nm
Excitation
570 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 0.5 mg/mlDMSO: 0.5 mg/mlEthanol: 0.5 mg/mlPBS (pH 7.2): 5 mg/ml
λmax
228, 288, 379, 610 nm
SMILES
O=C1C=C2OC3=CC([O-])=CC=C3[N+]([O-])=C2C=C1.[Na+]
InChi Code
InChI=1S/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1
InChi Key
IVGPGQSSDLDOLH-UHFFFAOYSA-M
Origin
Synthetic
Shipping & Storage Information
Storage
Room temperature
Shipping
Room temperature in continental US; may vary elsewhere
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    5 g

    $74

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    10 g

    $140

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    25 g

    $277

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    Product Description

    Resazurin is a blue non-fluorescent dye that when reduced to the highly red-fluorescent product resorufin (ex/em max = 570/580 nm, respectively) can be used as a quantifiable detection agent for enzyme activity assays.1,2 The non-fluorescent resazurin can be used as an oxidation-reduction indicator in cell viability assays in a variety of cells.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. O'Brien, J., Wilson, I., Orton, T., et al. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267(17), 5421-5426 (2000).

    2. Mayer, R.T., Jermyn, J.W., Burke, M.D., et al. Methoxyresorufin as a substrate for the fluorometric assay of insect microsomal O-dealkylases. Pestic. Biochem. Physiol. 7(4), 349-354 (1977).