A selective antibiotic used in cell culture systems
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Tetracycline (hydrochloride)

Item No. 14328

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(4S,4aS,5aS,6S,12aS)- 4-(dimethylamino)-1,4,4a,5,5a,6,11,12a-octahydro-3,6,10,12,12a-pentahydroxy-6-methyl-1,11-dioxo-2-naphthacenecarboxamide, monohydrochloride
CAS Number
64-75-5
Molecular Formula
C22H24N2O8 • HCl
Formula Weight
480.9
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 1 mg/mlDMSO: 1 mg/mlPBS (pH 7.2): 2 mg/ml
λmax
220, 268, 348, 361 nm
SMILES
O=C1C(C(N)=O)=C(O)[C@@H](N(C)C)[C@]2([H])C[C@](C3=C(O)[C@@]21O)([H])[C@@](O)(C)C4=CC=CC(O)=C4C3=O.Cl
InChi Code
InChI=1S/C22H24N2O8.ClH/c1-21(31)8-5-4-6-11(25)12(8)16(26)13-9(21)7-10-15(24(2)3)17(27)14(20(23)30)19(29)22(10,32)18(13)28;/h4-6,9-10,15,25,27-28,31-32H,7H2,1-3H3,(H2,23,30);1H/t9-,10-,15-,21+,22-;/m0./s1
InChi Key
XMEVHPAGJVLHIG-FMZCEJRJSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    Tetracycline is a broad-spectrum antibiotic that prevents bacterial growth by inhibiting protein synthesis. It binds to a single site in the 30S ribosomal subunit which prevents attachment of aminoacyl tRNA to the ribosomal acceptor site.1 It is used in cell biology as a selective agent in cell culture systems. Tetracycline is toxic to prokaryotic and eukaryotic cells and selects for cells harboring the bacterial tetR gene, which are resistant to the antibiotic.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wilson, D.N. The A-Z of bacterial translation inhibitors. Crit. Rev. Biochem. Mol. Biol. 44(6), 393-433 (2009).

    2. Degenkolb, J., Takahashi, M., Ellestad, G.A., et al. Structural requirements of tetracycline-Tet repressor interaction: Determination of equilibrium binding constants for tetracycline analogs with the Tet repressor. Antimicrob. Agents Chemother. 35(8), 1591-1595 (1991).