An odorless reducing agent
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Product Type

Biochemicals
Lab Reagents

Research Area

TCEP (hydrochloride)

Item No. 14329

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,3',3''-phosphinylidynetris-propanoic acid, monohydrochloride
CAS Number
51805-45-9
Synonyms
  • Tris(2-carboxyethyl)phosphine
Molecular Formula
C9H15O6P • HCl
Formula Weight
286.7
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 2 mg/mlDMSO: 3 mg/mlPBS (pH 7.2): 5 mg/ml
SMILES
OC(CCP(CCC(O)=O)CCC(O)=O)=O.Cl
InChi Code
InChI=1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H
InChi Key
PBVAJRFEEOIAGW-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

    $49

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    Product Description

    TCEP is an odorless, selective, and water-soluble reducing agent that is commonly used in many laboratory applications. It is commonly used to rapidly reduce protein and peptide disulfide bonds. TCEP can be combined with proteases to simultaneously reduce and digest proteins prior to mass spectrometry in order to dramatically increase sequence coverage.1 It has also been used to measure ascorbic acid and dehydroascorbic acid in biological samples.2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhang, H.M., McLoughlin, S.M., Frausto, S.D., et al. Simultaneous reduction and digestion of proteins with disulfide bonds for hydrogen/deuterium exchange monitored by mass spectrometry. Anal. Chem. 82(4), 1450-1454 (2010).

    2. Lykkesfeldt, J. Determination of ascorbic acid and dehydroascorbic acid in biological samples by high-performance liquid chromatography using subtraction methods: Reliable reduction with tris[2-carboxyethyl]phosphine hydrochloride. Anal. Biochem. 282(1), 89-93 (2000).

    3. Sato, Y., Uchiki, T., Iwama, M., et al. Determination of dehydroascorbic acid in mouse tissues and plasma by using tris(2-carboxyethyl)phosphine hydrochloride as reductant in metaphosphoric acid/ethylenediaminetetraacetic acid solution. Biol. Pharm. Bull. 33(3), 364-369 (2010).