A protein stain
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Ponceau S (sodium salt)

Item No. 14330

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3-hydroxy-4-[2-[2-sulfo-4-[2-(4-sulfophenyl)diazenyl]phenyl]diazenyl]-2,7-naphthalenedisulfonic acid, tetrasodium salt
CAS Number
6226-79-5
Synonyms
  • Acid Red 112
Molecular Formula
C22H12N4O13S4 • 4Na
Formula Weight
760.6
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 0.5 mg/mlDMSO: 10 mg/mlEthanol: 0.5 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
206, 239, 315, 357, 530 nm
SMILES
[O-]S(C(C=C1)=CC=C1/N=N/C2=CC=C(/N=N/C3=C(O)C(S([O-])(=O)=O)=CC4=C3C=CC(S([O-])(=O)=O)=C4)C(S([O-])(=O)=O)=C2)(=O)=O.[Na+].[Na+].[Na+].[Na+]
InChi Code
InChI=1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-2
InChi Key
KQHKSGRIBYJYFX-WNDMICSESA-J
Origin
Synthetic
Shipping & Storage Information
Storage
Room temperature
Shipping
Room temperature in continental US; may vary elsewhere
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    25 g

    $41

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    50 g

    $75

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    100 g

    $110

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    Product Description

    Ponceau S is a stain that permits the rapid, reversible detection of proteins immobilized on various matrices, including nitrocellulose and polyvinylidene fluoride membranes.1 It can be used to locate proteins or evaluate efficiency of transfer. Reversible staining with ponceau S has also been validated as an alternative to actin probing as a loading control.2 This stain can also be used as a fiducial marker in mass spectrometric imaging.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yonan, C.R., Duong, P.T., and Chang, F.N. High-efficiency staining of proteins on different blot membranes. Anal. Biochem. 338(1), 159-161 (2005).

    2. Romero-Calvo, I., Ocón, B., Martínez-Moya, P., et al. Reversible Ponceau staining as a loading control alternative to actin in Western blots. Anal. Biochem. 401(2), 318-320 (2010).

    3. Chughtai, K., Jiang, L., Greenwood, T.R., et al. Fiducial markers for combined 3-dimensional mass spectrometric and optical tissue imaging. Anal. Chem. 84(4), 1817-1823 (2012).