A dye used to stain proteins for IEF and SDS-PAGE
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Product Categories

Product Type

Biochemicals
Labeling & Detection
Fluorescent Probes

Research Area

Application

Electrophoresis
Fluorescence

Fast Green FCF

Item No. 14335

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-ethyl-N-[4-[[4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl](4-hydroxy-2-sulfophenyl)methylene]-2,5-cyclohexadien-1-ylidene]-3-sulfo-benzenemethanaminium, disodium salt
CAS Number
2353-45-9
Synonyms
  • NSC 379443
Molecular Formula
C37H34N2O10S3 • 2Na
Formula Weight
808.9
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 0.5 mg/mlDMSO: 10 mg/mlEthanol: 0.5 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
202, 306, 438, 616 nm
SMILES
O=S(C(C=C(O)C=C1)=C1/C(C2=CC=C(N(CC)CC3=CC(S([O-])(=O)=O)=CC=C3)C=C2)=C(C=C/4)/C=CC4=[N+](CC)/CC5=CC=CC(S([O-])(=O)=O)=C5)([O-])=O.[Na+].[Na+]
InChi Code
InChI=1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3,41,42,43,44,45,46,47,48,49);;
InChi Key
RZSYLLSAWYUBPE-UHFFFAOYSA-L
Origin
Synthetic
Shipping & Storage Information
Storage
Room temperature
Shipping
Room temperature in continental US; may vary elsewhere
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    5 g

    $42

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    10 g

    $79

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    25 g

    $161

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    100 g

    $561

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    Product Description

    Fast Green FCF is a dye used to stain proteins for IEF (isoelectric focusing) and SDS-PAGE. When bound to proteins, Fast Green FCF fluoresces near infrared (absorption maximum: 624 nm).1 Fast Green FCF staining is linear over a wider range of protein concentrations compared to Brilliant Blue R.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).