A fluorescent cationic dye useful for cell cycle determination
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Acridine Orange

Item No. 14338

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N3,N3,N6,N6-tetramethyl-3,6-acridinediamine
CAS Number
494-38-2
Synonyms
  • C.I. 46005B
  • NSC 194350
Molecular Formula
C17H19N3
Formula Weight
265.4
Emission
525 nm (dsDNA), 650 nm (ssDNA and RNA), 590 nm (low pH)
Excitation
502 nm (dsDNA), 460 nm (ssDNA and RNA), 475 nm (low pH)
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 2 mg/mlDMSO: 20 mg/mlEthanol: 0.3 mg/mlPBS (pH 7.2): 1 mg/ml
SMILES
CN(C)C1=CC(N=C(C=C(N(C)C)C=C2)C2=C3)=C3C=C1
InChi Code
InChI=1S/C17H19N3/c1-19(2)14-7-5-12-9-13-6-8-15(20(3)4)11-17(13)18-16(12)10-14/h5-11H,1-4H3
InChi Key
DPKHZNPWBDQZCN-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    5 g

    $69

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    10 g

    $115

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    25 g

    $263

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    Product Description

    Acridine orange is a cell-permeable, nucleic acid-selective fluorescent cationic dye useful for cell cycle determination and detection of cellular autophagy.1,2,3 It exhibits excitation/emission spectra of 502/525, 460/650, and 475/590 nm when bound to dsDNA, bound to ssDNA or RNA, and under acidic conditions, respectively.1,2 The ratio of acridine orange emission at 590 to emission at 525 nm can be used to quantify the increase in the number of acidic vesicular organelles observed during cellular autophagy.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Virant-Klun, I., Tomazevic, T., and Meden-Vrtovec, H. Sperm single-stranded DNA, detected by acridine orange staining, reduces fertilization and quality of ICSI-derived embryos. J. Assist. Reprod. Genet. 19(7), 319-328 (2002).

    2. Han, J., and Burgess, K. Fluorescent indicators for intracellular pH. Chem. Revs. 110(5), 2709-2728 (2010).

    3. Thomé, M.P., Filippi-Chiela, E.C., Villodre, E.S., et al. Ratiometric analysis of Acridine Orange staining in the study of acidic organelles and autophagy. J. Cell Sci. 129(24), 4622-4632 (2016).