A fluorogenic caspase subsrate
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Ac-DEVD-AFC

Item No. 14459

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine
CAS Number
201608-14-2
Synonyms
  • N-Acetyl-Asp-Glu-Val-Asp-7-amino-4-Trifluoromethylcoumarin
  • Caspase-3 Substrate (Fluorogenic)
Molecular Formula
C30H34F3N5O13
Formula Weight
729.6
Purity
≥98%
Emission
505 nm
Excitation
400 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
Water: 1 mg/ml
SMILES
CC(N[C@@H](CC(O)=O)C(N[C@H](C(N[C@@H](C(C)C)C(N[C@@H](CC(O)=O)C(NC1=CC(OC(C=C2C(F)(F)F)=O)=C2C=C1)=O)=O)=O)CCC(O)=O)=O)=O
InChi Code
InChI=1S/C30H34F3N5O13/c1-12(2)25(38-26(47)17(6-7-21(40)41)36-28(49)18(10-22(42)43)34-13(3)39)29(50)37-19(11-23(44)45)27(48)35-14-4-5-15-16(30(31,32)33)9-24(46)51-20(15)8-14/h4-5,8-9,12,17-19,25H,6-7,10-11H2,1-3H3,(H,34,39)(H,35,48)(H,36,49)(H,37,50)
InChi Key
GZDRODOYEFEHGG-NUDCOPPTSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $55

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    5 mg

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    10 mg

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    Product Description

    During apoptosis, activated caspase-3 cleaves several substrates, including poly(ADP-ribose) polymerase, which it specifically targets at the amino sequence DEVD.1 Ac-DEVD-AFC is a fluorogenic substrate for activated caspase-3 (Km = 9.7 µM), as well as related caspases.2,3,4 Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lazebnik, Y.A., Kaufmann, S.H., Desnoyers, S., et al. Cleavage of poly(ADP-ribose) polymerase by a proteinase with properties like ICE. Nature 371(6495), 346-347 (1994).

    2. Xiang, J., Chao, D.T., and Korsmeyer, S.J. BAX-induced cell death may not require interleukin 1β-converting enzyme-like proteases. Proc. Natl. Acad. Sci. USA 93(25), 14559-14563 (1996).

    3. Chandler, J.M., Cohen, G.M., and MacFarlane, M. Different subcellular distribution of caspase-3 and caspase-7 following Fas-induced apoptosis in mouse liver. The Journal of Biological Chemisty 273(18), 10815-10818 (1998).

    4. Marcelli, M., Cunningham, G.R., Haidacher, S.J., et al. Caspase-7 is activated during lovastatin-induced apoptosis of the prostate cancer cell line LNCaP. Cancer Res. 58(1), 76-83 (1998).