A fluorescent probe for dsRNA
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Pyronin Y

Item No. 14488

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,6-bis(dimethylamino)-xanthylium, monochloride
CAS Number
92-32-0
Synonyms
  • C.I. 45005
Molecular Formula
C17H19N2O • Cl
Formula Weight
302.8
Emission
560-580 nm
Excitation
540-550 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 3 mg/mlDMSO: 10 mg/mlPBS (pH 7.2): 10 mg/ml
SMILES
C/[N+](C)=C1C=C2OC3=CC(N(C)C)=CC=C3C=C2C=C/1.[Cl-]
InChi Code
InChI=1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1
InChi Key
INCIMLINXXICKS-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

    $36

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    5 g

    $70

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    10 g

    $120

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    Product Description

    Pyronin Y is a fluorescent probe which stains double stranded RNA in living or fixed cells as well as in tissues.1,2 When used in living cell preparations, it is commonly combined with 50-100 µM verapamil to prevent efflux of the dye. It has been used to ascertain the cell cycle state of stem cells and is amenable to flow cytometry.3,4 Maximum excitation is at 540-550 nm, with maximum emission at 560-580 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Andrews, L.M., Jones, M.R., Digman, M.A., et al. Spectral phasor analysis of Pyronin Y labeled RNA microenvironments in living cells. Biomed. Opt. Express 4(1), 171-177 (2013).

    2. Mohtasham, N., Mahdavi-Shahri, N., Salehinejad, J., et al. Detection of nucleoproteins in squamous cell carcinoma, and dysplastic and normal mucosa in the oral cavity by methyl green-pyronin staining. J. Oral Sci. 52(2), 239-243 (2010).

    3. Challen, G.A., Boles, N., Lin, K.K.L., et al. Mouse hematopoietic stem cell indentification and analysis. Cytometry A 75(1), 14-24 (2013).

    4. Tanke, H.J., Rothbarth, P.H., Vossen, J.M., et al. Flow cytometry of reticulocytes applied to clinical hematology. Blood 61(6), 1091-1097 (1983).