Active, pure human recombinant enzyme
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MIF (human, recombinant)

Item No. 14493

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Macrophage Migration Inhibitory Factor
Purity
≥95% estimated by SDS-PAGE
Source
Recombinant human MIF expressed in E. coli
MW
12.6 kDa
Formulation (Request formulation change)
50 mM Tris pH 7.5, 150 mM sodium chloride
UniProt Accession №
P14174
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $489

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    Product Description

    Macrophage Migration Inhibitory Factor (MIF) is a small, pro-inflammatory cytokine. MIF signaling is important in septic shock, arthritis, colitis, and cardiovascular diseases.1 MIF counteracts glucocorticoid signaling, which would otherwise inhibit inflammation. Secreted MIF binds to CD74 receptors, which then couple to CD44, during monocyte recruitment.2 In addition to CD44 binding, MIF is an enzymatically active keto-enol tautomerase that is inhibited by isothiocyanates such as sulforaphane.3 Overexpression of MIF has been seen in several cancers. MIF overexpression has been shown to promote hypoxia induced HIF-1α stabilization leading to changes in the tumor microenvironment, including stimulation of angiogenesis and neovascularization.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zernecke, A., Bernhagen, J., and Weber, C. Macrophage migration inhibitory factor in cardiovascular disease. Circulation 117(12), 1594-1602 (2008).

    2. Leng, L., and Bucala, R. Insight into the biology of macrophage migration inhibitory factor (MIF) revealed by the cloning of its cell surface receptor. Cell Res. 16(2), 162-168 (2006).

    3. Healy, Z.R., Liu, H., Holtzclaw, W.D., et al. Inactivation of tautomerase activity of macrophage migration inhibitory factor by sulforaphane: A potential biomarker for anti-inflammatory intervention. Cancer Epidemiol. Biomarkers Prev. 20(7), 1516-1523 (2011).

    4. Rendon, B.E., Willer, S.S., Zundel, W., et al. Mechanisms of macrophage migration inhibitory factor (MIF)-dependent tumor microenvironmental adaptation. Exp. Mol. Pathol. 86(3), 180-185 (2009).