A dye for staining proteins
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Coomassie Blue R-250

Item No. 14500

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[4-[[4-[(4-ethoxyphenyl)amino]phenyl][4-[ethyl[(3-sulfophenyl)methyl]amino]phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-N-ethyl-3-sulfo-benzenemethanaminium, monosodium salt
CAS Number
6104-59-2
Synonyms
  • C.I. 42660
Molecular Formula
C45H44N3O7S2 • Na
Formula Weight
826.0
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 2 mg/mlDMSO: 10 mg/mlEthanol: 0.1 mg/mlPBS (pH 7.2): 1 mg/ml
SMILES
CC/[N+](CC1=CC(S([O-])(=O)=O)=CC=C1)=C(C=C/2)\C=CC2=C(C3=CC=C(N(CC)CC4=CC=CC(S([O-])(=O)=O)=C4)C=C3)\C(C=C5)=CC=C5NC6=CC=C(OCC)C=C6.[Na+]
InChi Code
InChI=1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53
InChi Key
NKLPQNGYXWVELD-UHFFFAOYSA-M
Origin
Synthetic
Shipping & Storage Information
Storage
Room temperature
Shipping
Room temperature in continental US; may vary elsewhere
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    10 g

    $36

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    25 g

    $62

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    50 g

    $110

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    100 g

    $186

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    Product Description

    Coomassie blue R-250 is a dye that is commonly used in laboratories to stain or quantify proteins. It is a sensitive stain for protein detection in polyacrylamide gels, typically giving blue bands on a clear background with a sensitivity of 50-100 ng/band. It may be combined with other stains, such as silver stain, to distinguish different types of proteins.1 Coomassie blue R-250 displays metachromasia by appearing pink-red, rather than blue, when binding certain proteins, such as collagen and histone H1.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Dzandu, J.K., Deh, M.E., Barratt, D.L., et al. Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique. Proc. Natl. Acad. Sci. USA 81(6), 1733-1737 (1984).

    2. Hattori, S., Sakai, K., Watanabe, K., et al. The induction of metachromasia and circular dichroism of Coomassie Brilliant Blue R-250 with collagen and histone H1 is due to the low content of hydrophobic amino acid residues in these proteins. J. Biochem. 119(3), 400-406 (1996).