A fluorogenic probe for H2S
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SF7-AM

Item No. 14623

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[(3',6'-diazido-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)carbonyl]-glycine, (acetyloxy)methyl ester
CAS Number
1416872-50-8
Synonyms
  • Sulfidefluor-7 acetoxymethyl ester
Molecular Formula
C31H23N7O12
Formula Weight
685.6
Purity
≥95%
Emission
526 nm
Excitation
498 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlDMF:PBS (pH 7.2) (1:1): 0.5 mg/mlDMSO: 5 mg/ml
λmax
234 nm
SMILES
O=C(O1)C2=C(C=CC(C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=O)=C2)C31C4=C(C=C(N=[N+]=[N-])C=C4)OC5=C3C=CC(N=[N+]=[N-])=C5
InChi Code
InChI=1S/C31H23N7O12/c1-16(39)45-14-47-27(41)12-38(13-28(42)48-15-46-17(2)40)29(43)18-3-6-22-21(9-18)30(44)50-31(22)23-7-4-19(34-36-32)10-25(23)49-26-11-20(35-37-33)5-8-24(26)31/h3-11H,12-15H2,1-2H3
InChi Key
QIAZHSBWGCYJGQ-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

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    Product Description

    SF7-AM is a cell-trappable fluorogenic probe for the detection of hydrogen sulfide (H2S).1 Following entry into cells, cleavage of the acetoxymethyl ester groups by intracellular esterases results in an anionic charge, trapping the probe inside the cells.2 Reaction of the azide moieties with H2S in buffers or live cells leads to generation of carboxamide rhodamine 110, which displays excitation/emission maxima of 498/526 nm, respectively.1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lin, V.S., Lippert, A.R., and Chang, C.J. Cell-trappable fluorescent probes for endogenous hydrogen sulfide signaling and imaging H2O2-dependent H2S production. Proc. Natl. Acad. Sci. USA 110(18), 7131-7135 (2013).

    2. Lippert, A.R. Designing reaction-based fluorescent probes for selective hydrogen sulfide detection. J. Inorg. Biochem. 133, 136-142 (2014).

    Product Citations

    Jeong, Y.S., Park, B., Lee, M., et al. Hydrogen sulfide induces regulatory B cells via glycolysis and mitochondrial ROS, attenuating LPS-induced lung injury. Cell Commun. Signal. 23(1), 446 (2025).

    Petrovic, D., Slade, L., Paikopoulos, Y., et al. Ergothioneine improves healthspan of aged animals by enhancing cGPDH activity through CSE-dependent persulfidation. Cell Metab. 37(2), 524-556 (2025).