Active • Host: Insect cells • AA: 57-454 • Tag: C-terminal His • MW: 48.4 kDa
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BACE1 (human, recombinant)

Item No. 14645

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • β-site Amyloid Precursor Protein Cleaving Enzyme 1
  • ASP-2
  • BACE
  • Memapsin 2
  • Membrane-bound Aspartic Protease
  • β-secretase 1
Purity
≥85% estimated by SDS-PAGE
Source
Active recombinant human C-terminal His-tagged BACE1 expressed in insect cells
Amino Acids
57-454
MW
48.4 kDa
Formulation (Request formulation change)
50 mM HEPES, pH 7.4, with 150 mM sodium chloride and 20% glycerol
UniProt Accession №
P56817
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $341

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    Product Description

    β-Secretase 1 (BACE1) is an aspartic protease and member of the peptidase A1 protease family.1 BACE1 is transcribed as a preprotein composed of an N-terminal signal peptide, a pro-peptide, a catalytic domain, a transmembrane domain, and a cytosolic domain. The N-terminal signal peptide traffics the BACE1 preprotein to the endoplasmic reticulum where furin-mediated cleavage of the propeptide produces mature BACE1. The transmembrane domain of mature BACE1 drives localization to the Golgi where BACE1 is post-translationally activated and functions at endosomal or Golgi membranes.1,2 BACE1 is expressed primarily in the brain and at lower levels in pancreatic β-cells, adipocytes, hepatocytes, and vascular cells. BACE1 cleaves amyloid precursor protein (APP) to initiate the generation of amyloid-β (Aβ), and BACE1 knockout rescues memory deficits and cholinergic dysfunction in transgenic mouse models of Alzheimer's disease.3,4 Additional BACE1 substrates include seizure-related 6 (SEZ6) and seizure-related 6-like (SEZ6L), and BACE1-mediated cleavage of Sez6 and Sez6L is increased in the brain in a mouse model of Niemann-Pick type C disease.5 Cayman's BACE1 (human, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Taylor, H.A., Przemylska, L., Clavane, E.M., et al. BACE1: More than just a β-secretase. Obes. Rev. 23(7), e13430 (2022).

    2. Araki, W. Post-translational regulation of the β-secretase BACE1. Brain Res. Bull. 126 (Pt. 2), 170-177 (2016).

    3. Yan, R., Fan, Q., Zhou, J., et al. Inhibiting BACE1 to reverse synaptic dysfunctions in Alzheimer’s disease. Neurosci. Biobehav. Rev. 65, 326-340 (2016).

    4. Pietrak, B.L., Crouthamel, M.C., Tugusheva, K., et al. Biochemical and cell-based assays for characterization of BACE-1 inhibitors. Anal. Biochem. 342(1), 144-151 (2005).

    5. Causevic, M., Dominko, K., Malnar, M., et al. BACE1-cleavage of Sez6 and Sez6L is elevated in Niemann-Pick type C disease mouse brains. PLoS One 13(7), e0200344 (2018).