A colorimetric probe for free radical scavengers
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DPPH

Item No. 14805

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,2-diphenyl-1-(2,4,6-trinitrophenyl)-hydrazinyl
CAS Number
1898-66-4
Synonyms
  • 2,2-Diphenyl-1-Picrylhydrazyl
  • DPPH radical
Molecular Formula
C18H12N5O6
Formula Weight
394.3
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 10 mg/mlEthanol: 10 mg/ml
λmax
327, 515 nm
SMILES
O=[N+](C1=C(C([N+]([O-])=O)=CC([N+]([O-])=O)=C1)[N]N(C2=CC=CC=C2)C3=CC=CC=C3)[O-]
InChi Code
InChI=1S/C18H12N5O6/c24-21(25)15-11-16(22(26)27)18(17(12-15)23(28)29)19-20(13-7-3-1-4-8-13)14-9-5-2-6-10-14/h1-12H
InChi Key
HHEAADYXPMHMCT-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 mg

    $50

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    100 mg

    $95

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    500 mg

    $347

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    Product Description

    2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a stable free radical and colorimetric probe for the detection of free radical scavengers.1,2 Upon reaction with antioxidants, DPPH turns from deep violet to yellow, which can be quantified by colorimetric detection at 515 nm as a measure of antioxidant capacity.3 DPPH has commonly been used to measure the antioxidant potential of plant extracts, foodstuffs, and pure compounds.1,2,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kedare, S.B., and Singh, R.P. Genesis and development of DPPH method of antioxidant assay. J. Food Sci. Technol. 48(4), 412-422 (2011).

    2. Pyrzynska, K., and Pekal, A. Application of free radical diphenylpicrylhydrazyl (DPPH) to estimate the antioxidant capacity of food samples. Anal. Methods 5, 4288-4295 (2013).

    3. Molyneux, P. The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 26(2), 211-219 (2004).

    4. Idhayadhulla, A., Xia, L., Lee, Y.R., et al. Synthesis of novel and diverse mollugin analogues and their antibacterial and antioxidant activities. Bioorg. Chem. 52, 77-82 (2014).

    Product Citations

    Ricotta, M., Iannuzzi, M., De Vivo, G., et al. Physio-pathological roles of transglutaminase-catalyzed reactions. World J. Biol. Chem. 1(5), 181-187 (2010).

    Nagakannan, P., Islam, M.I., Conrad, M., et al. Cathepsin B is an excutioner of ferroptosis. Biochim. Biophys. Acta Mol. Cell Res. 1868(3), 118928 (2021).

    Zou, Y., Henry, W.S., Ricq, E.L., et al. Plasticity of ether lipids promotes ferroptosis susceptibility and evasion. Nature 585(7826), 603-608 (2020).