A cell-impermeable, negatively charged tetrazolium dye
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XTT (sodium salt hydrate)

Item No. 14919

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium, inner salt, monosodium salt, hydrate
CAS Number
413585-64-5
Molecular Formula
C22H16N7O13S2 • Na [XH2O]
Formula Weight
650.5
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 0.5 mg/mlDMSO: 3 mg/mlPBS (pH 7.2): 3 mg/ml
λmax
284 nm
SMILES
COC(C=C([N+]([O-])=O)C(S([O-])(=O)=O)=C1)=C1N2N=C(C(NC3=CC=CC=C3)=O)N=[N+]2C4=CC(S([O-])(=O)=O)=C([N+]([O-])=O)C=C4OC.[Na+].O
InChi Code
InChI=1S/C22H17N7O13S2.Na.H2O/c1-41-17-8-15(28(31)32)19(43(35,36)37)10-13(17)26-24-21(22(30)23-12-6-4-3-5-7-12)25-27(26)14-11-20(44(38,39)40)16(29(33)34)9-18(14)42-2;;/h3-11H,1-2H3,(H2-,23,30,35,36,37,38,39,40);;1H2/q;+1;/p-1
InChi Key
OMYYRPUFIRZPOS-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    25 mg

    $62

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    50 mg

    $109

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    100 mg

    $171

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    250 mg

    $365

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    Product Description

    XTT is a cell-impermeable, negatively charged tetrazolium dye that produces a water-soluble formazan when reduced at the cell surface by cellular-derived NADH and an electron mediator.1,2 It is frequently used in colorimetric assays to measure cell proliferation, cytotoxicity, and apoptosis.3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Berridge, M.V., Tan, A.S., and Herst, P.M. Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction. Biotechnol. Ann. Rev. 11, 127-152 (2005).

    2. Berridge, M.V., and Tan, A.S. Trans-plasma membrane electron transport: A cellular assay for NADH-and NADPH-oxidase based on extracellular, superoxide-mediated reduction of the sulfonated tetazolium salt WST-1. Protoplasma 205, 74-82 (1998).

    3. Sutherland, M.W., and Learmonth, B.A. The tetrazolium dyes MTS and XTT provide new quantitative assays for superoxide and superoxide dismutase. Free Radic. Res. 27(3), 283-289 (1997).