A non-ionic detergent
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Biochemicals
Lab Reagents
Detergents
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Digitonin

Item No. 14952

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
(2α,3β,5α,15β,25R)-2,15-dihydroxyspirostan-3-yl O-β-D-glucopyranosyl-(1→3)-O-β-D-galactopyranosyl-(1→2)-O-[β-D-xylopyranosyl-(1→4)-β-D-galactopyranoside
CAS Number
11024-24-1
Synonyms
  • Digitin
  • NSC 23471
  • NSC 237417
Molecular Formula
C56H92O29
Formula Weight
1229.3
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 30 mg/ml
SMILES
C[C@@]12[C@@]([H])([C@H](O)[C@@]3([H])[C@]2([H])[C@H](C)[C@]4(OC[C@H](C)CC4)O3)[C@@](CC[C@]5([H])[C@@]6(C[C@@H](O)[C@H](O[C@]([C@@H]([C@@H](O)[C@H]7O[C@@](O[C@H](CO)[C@@H](O)[C@@H]8O[C@@](OC[C@@H](O)[C@@H]9O)([H])[C@@H]9O)([H])[C@@H]8O[C@@](O[C@H](CO)[C@H](O)[C@@H]%10O[C@]([C@@H]([C@@H](O)[C@@H]%11O)O)([H])O[C@@H]%11CO)([H])[C@@H]%10O)O)([H])O[C@@H]7CO)C5)C)([H])[C@]6([H])CC1
InChi Code
InChI=1S/C56H92O29/c1-19-7-10-56(75-17-19)20(2)31-45(85-56)37(67)32-22-6-5-21-11-26(24(61)12-55(21,4)23(22)8-9-54(31,32)3)76-50-42(72)39(69)44(30(16-60)80-50)81-53-48(47(36(66)29(15-59)79-53)83-49-40(70)33(63)25(62)18-74-49)84-52-43(73)46(35(65)28(14-58)78-52)82-51-41(71)38(68)34(64)27(13-57)77-51/h19-53,57-73H,5-18H2,1-4H3/t19-,20+,21+,22-,23+,24-,25-,26-,27-,28-,29-,30-,31+,32-,33+,34-,35+,36-,37+,38+,39-,40-,41-,42-,43-,44+,45-,46+,47+,48-,49+,50-,51+,52+,53+,54-,55+,56-/m1/s1
InChi Key
UVYVLBIGDKGWPX-KUAJCENISA-N
Origin
Plant/Digitalis purpurea
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 mg

    $83

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    250 mg

    $186

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    500 mg

    $330

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    1 g

    $537

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    Product Description

    Digitonin is a glycoside obtained from D. purpurea that is used as a non-ionic detergent to solubilize membrane-bound proteins, to precipitate cholesterol, and to permeabilize cell membranes.1,2,3

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Nazari, M., Kurdi, M., and Heerklotz, H. Classifying surfactants with respect to their effect on lipid membrane order. Biophys. J. 102(3), 498-506 (2012).

    2. Gardner, J.A., Gainsborough, H., and Murray, R. Studies in the cholesterol content of normal human plasma: An improved macromethod for the estimation of cholesterol by digitonin. Biochem. J. 32(1), 15-18 (1938).

    3. Knight, D.E., and Scrutton, M.C. Gaining access to the cytosol: The technique and some applications of electropermeabilization. Biochem. J. 234(3), 497-506 (1986).

    Product Citations

    Gehne, N., Lamik, A., Lehmann, M., et al. Cross-over endocytosis of claudins is mediated by interactions via their extracellular loops. PLoS One 12(8), e0182106 (2017).