A caspase-3 fluorogenic substrate
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Ac-DEVD-AMC

Item No. 14986

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-acetyl-L-α-aspartyl-L-α-glutamyl-L-valyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-α-asparagine
CAS Number
169332-61-0
Synonyms
  • N-Acetyl-Asp-Glu-Val-Asp-7-amido-4-Methylcoumarin
  • Caspase-3 Substrate (Fluorogenic)
Molecular Formula
C30H37N5O13
Formula Weight
675.6
Purity
≥95%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: 10 mg/ml*
SMILES
CC(C1=CC=C(C=C1O2)NC([C@@H](NC([C@@H](NC([C@@H](NC([C@H](CC(O)=O)NC(C)=O)=O)CCC(O)=O)=O)C(C)C)=O)CC(O)=O)=O)=CC2=O
InChi Code
InChI=1S/C30H37N5O13/c1-13(2)26(35-27(44)18(7-8-22(37)38)33-29(46)19(11-23(39)40)31-15(4)36)30(47)34-20(12-24(41)42)28(45)32-16-5-6-17-14(3)9-25(43)48-21(17)10-16/h5-6,9-10,13,18-20,26H,7-8,11-12H2,1-4H3,(H,31,36)(H,32,45)(H,33,46)(H,34,47)(H,35,44)(
InChi Key
ALZSTTDFHZHSCA-RNVDEAKXSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $68

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    5 mg

    $179

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    10 mg

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    Product Description

    Ac-DEVD-AMC is a fluorogenic substrate that can be cleaved specifically by caspase-3.1 Caspase activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kravchenko, D.V., Kysil, V.V., Ilyn, A.P., et al. 1,3-Dioxo-4-methyl-2,3-dihydro-1H-pyrrolo[3,4-c]quinolines as potent caspase-3 inhibitors. Bioorg. Med. Chem. Lett. 15(7), 1841-1845 (2005).

    Product Citations

    Fennell, E.M.J., Aponte-Collazo, L.J., Wynn, J.D., et al. Characterization of TR-107, a novel chemical activator of the human mitochondrial protease ClpP. Pharmacol. Res. Perspect. 10(4), e00993 (2022).

    Nagakannan, P., Islam, M.I., Conrad, M., et al. Cathepsin B is an excutioner of ferroptosis. Biochim. Biophys. Acta Mol. Cell Res. 1868(3), 118928 (2021).