A caspase-6 fluorogenic substrate
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Ac-VEID-AMC (ammonium salt)

Item No. 14989

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-acetyl-L-valyl-L-α-glutamyl-L-isoleucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-α-asparagine, ammonium salt
Synonyms
  • N-Acetyl-Val-Glu-Ile-Asp-7-amido-4-Methylcoumarin
  • Caspase-6 Substrate (Fluorogenic)
Molecular Formula
C32H43N5O11 • XNH4
Formula Weight
673.7
Purity
≥95%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 10 mg/ml
λmax
328 nm
SMILES
CC(C1=CC=C(C=C1O2)NC([C@@H](NC(C(NC([C@@H](NC([C@@H](NC(C)=O)C(C)C)=O)CCC(O)=O)=O)[C@H](CC)C)=O)CC(O)=O)=O)=CC2=O.[NH4+]
InChi Code
InChI=1S/C32H43N5O11.H3N/c1-7-16(4)28(37-29(44)21(10-11-24(39)40)35-31(46)27(15(2)3)33-18(6)38)32(47)36-22(14-25(41)42)30(45)34-19-8-9-20-17(5)12-26(43)48-23(20)13-19;/h8-9,12-13,15-16,21-22,27-28H,7,10-11,14H2,1-6H3,(H,33,38)(H,34,45)(H,35,46)(H,36,47)(H,37,44)(H,39,40)(H,41,42);1H3/p+1/t16-,21-,22-,27-,28?;/m0./s1
InChi Key
QUKLKUUAACWSEY-QRUVTBKYSA-O
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $77

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    5 mg

    $192

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    10 mg

    $306

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    Product Description

    Ac-VEID-AMC is a fluorogenic substrate based on the caspase-6 cleavage site in lamin A at amino acids VEID during apoptosis.1 It has also been reported to be cleaved by related proteases, including caspase-8.2 Caspase activity can be quantified by fluorescent detection of free AMC (also known as 7-amino-4-methylcoumarin), which is excited at 340-360 nm and emits at 440-460 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Talanian, R.V., Quinlan, C., Trautz, S., et al. Substrate specificities of caspase family proteases. The Journal of Biological Chemisty 272(15), 9677-9682 (1997).

    2. Chae, H.J., Park, K.M., Lee, G.Y., et al. Je-Chun-Jun induced apoptosis of human cervical carcinoma HeLa cells. Acta Pharmacol. Sin. 25(10), 1372-1379 (2004).