A fluorogenic substrate for group I caspases
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Ac-WEHD-AFC (trifluoroacetate salt)

Item No. 14992

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-acetyl-L-tryptophyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-L-α-asparagine, trifluoroacetate salt
Synonyms
  • N-Acetyl-Trp-Glu-His-Asp-7-amino-4-Trifluoromethylcoumarin
  • Caspase-1 Substrate (Fluorogenic)
  • Caspase-5 Substrate (Fluorogenic)
Molecular Formula
C38H37F3N8O11 • XCF3COOH
Formula Weight
838.7
Purity
≥95%
Emission
505 nm
Excitation
400 nm
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMSO: 10 mg/mlWater: 1 mg/ml
λmax
210, 338 nm
SMILES
OC(C[C@H](NC([C@@H](NC([C@@H](NC([C@H](CC1=CNC2=C1C=CC=C2)NC(C)=O)=O)CCC(O)=O)=O)CC3=CNC=N3)=O)C(NC(C=C4O5)=CC=C4C(C(F)(F)F)=CC5=O)=O)=O.FC(F)(C(O)=O)F
InChi Code
InChI=1S/C38H37F3N8O11.C2HF3O2/c1-18(50)45-27(10-19-15-43-25-5-3-2-4-22(19)25)36(58)47-26(8-9-31(51)52)34(56)48-28(11-21-16-42-17-44-21)37(59)49-29(14-32(53)54)35(57)46-20-6-7-23-24(38(39,40)41)13-33(55)60-30(23)12-20;3-2(4,5)1(6)7/h2-7,12-13,15-17,26-29,43H,8-11,14H2,1H3,(H,42,44)(H,45,50)(H,46,57)(H,47,58)(H,48,56)(H,49,59)(H,51,52)(H,53,54);(H,6,7)/t26-,27-,28-,29-;/m0./s1
InChi Key
UELPUHYAUOLMOR-MROKUPSKSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $103

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    5 mg

    $253

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    10 mg

    $454

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    Product Description

    Ac-WEHD-AFC is a fluorogenic substrate that can be cleaved by group I caspases (caspase-1, -4, and -5).1 Caspase activity can be quantified by fluorescent detection of free AFC (also known as 7-amino-4-trifluoromethylcoumarin), which is excited at 400 nm and emits at 505 nm.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Park, K., Kuechle, M.K., Choe, Y., et al. Expression and characterization of constitutively active human caspase-14. Biochem. Biophys. Res. Commun. 347(4), 941-948 (2006).

    Product Citations

    Burkovetskaya, M., Bosch, M.E., Karpuk, N., et al. Caspase 1 activity influences juvenile Batten disease (CLN3) pathogenesis. J. Neurochem. 148(5), 652-668 (2019).