An indicator dye of mitochondrial transmembrane potential
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

JC-1

Item No. 15003

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5,6-dichloro-2-[3-(5,6-dichloro-1,3-diethyl-1,3-dihydro-2H-benzimidazol-2-ylidene)-1-propen-1-yl]-1,3-diethyl-1H-benzimidazolium, monoiodide
CAS Number
3520-43-2
Synonyms
  • CBIC2
  • NK 1420
Molecular Formula
C25H27Cl4N4 • I
Formula Weight
652.2
Purity
≥90%
Emission
535 nm (monomer), 570-610 nm (aggregate)
Excitation
485 nm (monomer), 520-570 nm (aggregate)
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 3 mg/mlDMSO: 1 mg/ml
λmax
514 nm
SMILES
CCN(C(C=C(Cl)C(Cl)=C1)=C1N/2CC)C2=C/C=C/C3=[N+](CC)C4=C(C=C(Cl)C(Cl)=C4)N3CC.[I-]
InChi Code
InChI=1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1
InChi Key
FYNNIUVBDKICAX-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $75

    Add

    5 mg

    $333

    Add

    10 mg

    $589

    Add
    Product Description

    JC-1 is a membrane-permeable cationic dye that is used to study mitochondrial integrity in the context of cellular apoptosis.1,2,3 It selectively enters mitochondria and changes fluorescence characteristics with alteration in mitochondrial transmembrane potential (ΔΨm). In healthy cells with a high mitochondrial ΔΨm, JC-1 forms complexes known as J-aggregates, which fluoresce red/orange and may be detected using FL2 settings by flow cytometry.3 A drop in ΔΨm, a very early event in apoptosis, results in JC-1 monomers, which fluoresce green (FL1 settings on flow cytometers).2,3 JC-1 may also be used with fluorescent microscopes or plate readers, using excitation at 520-570 nm and emission at 570-610 nm for J-aggregates and excitation at 485 nm and emission at 535 nm for monomers.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Abdalah, R., Wei, L., Francis, K., et al. Valinomycin-induced apoptosis in Chinese hamster ovary cells. Neurosci. Lett. 405(1-2), 68-73 (2006).

    2. Petit, P.X., Lecoeur, H., Zorn, E., et al. Alterations in mitochondrial structure and function are early events of dexamethasone-induced thymocyte apoptosis. J. Cell Biol. 130(1), 157-167 (1995).

    3. Salvioli, S., Ardizzoni, A., Franceschi, C., et al. JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess Δψ changes in intact cells: Implications for studies on mitochondrial functionality during apoptosis. FEBS Lett. 411(1), 77-82 (1997).

    Product Citations

    Grossini, E., Vebkatesan, S., Alkabes, M., et al. Membrane blue dual protects retinal pigment epithelium cells/ganglion cells-like through modulation of mitochondria function. Biomedicines 10(11), 2854 (2022).

    Ekaney, M.L., Gray, G.G., McKillop, I.H., et al. Enhanced platelet function in cold stored whole blood supplemented with resveratrol or cytochrome C. J. Trauma Acute Care Surg. 85(1), S92-S97 (2018).

    Bénardais, K., Kotsiari, A., Skuljec, J., et al. Cuprizone [bis(cyclohexylidenehydrazide)] is selectively toxic for mature oligodendrocytes. Neurotox. Res. 24(2), 244-250 (2013).

    Ayers, S., Graf, T.N., Adcock, A.F., et al. Resorcylic acid lactones with cytotoxic and NF-κB inhibitory activities and their structure-activity relationships. J. Nat. Prod. 74(5), 1126-1131 (2011).