Active, pure human recombinant enzyme
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Nicotinamide N-Methyltransferase (human, recombinant)

Item No. 15138

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • NNMT
Purity
≥95% estimated by SDS-PAGE
Source
Recombinant N-terminal His- and SUMO-tagged protein expressed in E. coli
Amino Acids
2-264 (full length)
MW
42.6 kDa
Formulation (Request formulation change)
50 mM Tris, pH 8.0, containing 500 mM sodium chloride, 5% glycerol, and 1 mM DTT
License
SUMOpro tag was used under non-exclusive license from LifeSensors, Inc.
UniProt Accession №
P40261
Shipping & Storage Information
Storage
-80°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $528

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    Product Description

    Nicotinamide N-Methyltransferase (NNMT) methylates nicotinamide and other pyridine containing compounds.1 NNMT transfers a methyl group from S-adenosylmethionine (SAM) to nitrogen N1 of nicotinamide to produce 1-methylnicotinamide (MNA) and S-adenosylhomocysteine. MNA is a messenger molecule that increases neurite branching, serves as an anti-thrombotic, and has anti-inflammatory properties.2,3,4 The role of NNMT overexpression in cancers may be to alter epigenetic methylation patterns in two ways: by lowering the intracellular concentration of SAM, required by methyltransferase enzymes, and by depleting the available NAD+ by transforming nicotinamide to MNA.4,5 Sirtuins use NAD+ as a substrate to alter protein acetylation and ribosylation, including histone targets.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Alston, T.A., and Abeles, R.H. Substrate specificity of nicotinamide methyltransferase isolated from porcine liver. Arch. Biochem. Biophys. 260(2), 601-608 (1988).

    2. Thomas, M.G., Saldanha, M., Mistry, R.J., et al. Nicotinamide N-methyltransferase expression in SH-SY5Y neuroblastoma and N27 mesencephalic neurones induces changes in cell morphology via ephrin-B2 and Akt signalling. Cell Death Dis. 4, e669 (2013).

    3. Chlopicki, S., Swies, J., Mogielnicki, A., et al. 1-Methylnicotinamide (MNA), a primary metabolite of nicotinamide, exerts anti-thrombotic activity mediated by a cyclooxygenase-2/prostacyclin pathway. Br. J. Pharmacol. 152(2), 230-239 (2007).

    4. Shlomi, T., and Rabinowitz, J.D. Cancer mistunes methylation. Nat. Chem. Biol. 9(5), 293-294 (2013).

    5. Ulanovskaya, O.A., Zuhl, A.M., and Cravatt, B.F. NNMT promotes epigenetic remodeling in cancer by creating a metabolic methylation sink. Nat. Chem. Biol. 9(5), 300-306 (2013).

    Product Citations

    Ailabouni, A.S., Vijaywargi, G., Subash, S., et al. Is N1-methylnicotinamide a good organic cation transporter 2 (OCT2) biomarker? Metabolites 15(2), 80 (2025).