An inducer of β-galactosidase activity
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IPTG (dioxane free)

Item No. 15300

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
1-methylethyl 1-thio-β-D-galactopyranoside
CAS Number
367-93-1
Synonyms
  • Isopropyl β-​thiogalactoside
Molecular Formula
C9H18O5S
Formula Weight
238.3
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 30 mg/mlDMSO: 20 mg/mlEthanol: 20 mg/mlPBS (pH 7.2): 10 mg/ml
SMILES
O[C@@H]1[C@H](O)[C@@H](O)[C@H](SC(C)C)O[C@@H]1CO
InChi Code
InChI=1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1
InChi Key
BPHPUYQFMNQIOC-NXRLNHOXSA-N
Origin
Synthetic
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

    $39

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    5 g

    $126

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    10 g

    $231

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    25 g

    $434

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    Product Description

    IPTG is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon.1 It is used in the concentration range of 100 μM - 1.5 mM to induce protein expression where the gene is under the control of the lac operator and is suitable for use with X-gal or bluo-gal to detect lac gene activity in cloning procedures.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kaster, K.R., Burgett, S.G., Rao, R.N., et al. Analysis of a bacterial hygromycin B resistance gene by transcriptional and translational fusions and by DNA sequencing. Nucleic Acids Res. 11(19), 6895-6911 (1983).

    2. Sambrook, J., and Russell, D.W. Molecular cloning: A laboratory manual. 3, 1-21 (2001).