Harringtonine

Harringtonine is a natural alkaloid that inhibits protein synthesis at low micromolar concentrations.¹ It immobilizes ribosomes immediately after the initiation of translation.^1,2 While harringtonine has drawn some interest in mitigating cancer, its homolog homoharrington is more effective, particularly for chronic myelogenous leukemia.^3,4,5 The ability of harringtonine to immobilize initiating ribosomes can be used to capture ribosome-protected mRNA fragments for evaluating translation.²

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1. Fresno, M., Jiménez, A., and Vázquez, D. Inhibition of translation in eukaryotic systems by harringtonine. Eur. J. Biochem. 72(2), 323-330 (1977).

2. Ingolia, N.T., Brar, G.A., Rouskin, S., et al. The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments. Nat. Protoc. 7(8), 1534-1550 (2012).

3. Kantarjian, H.M., O'Brien, S., Anderlini, P., et al. Treatment of chronic myelogenous leukemia: Current status and investigational options. Blood 87(8), 3069-3081 (1996).

4. Chen, Y., Peng, C., Sullivan, C., et al. Novel therapeutic agents against cancer stem cells of chronic myeloid leukemia. Anticancer Agents Med. Chem. 10(2), 111-115 (2010).

5. Robert, F., Carrier, M., Rawe, S., et al. Altering chemosensitivity by modulating translation elongation. PLoS One 4(5), e5428 (2009).