Host: E. coli • AA: 2-217 (full length) • Tag: N-terminal GST • MW: 51.8 kDa
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

eIF4E (human recombinant; GST-tagged)

Item No. 15564

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Eukaryotic Translation Initiation Factor 4E
  • mRNA Cap-binding Protein
Purity
≥85% estimated by SDS-PAGE
Source
Recombinant N-terminal GST-tagged protein expressed in E. coli
Amino Acids
2-217 (full length)
MW
51.8 kDa
Formulation (Request formulation change)
50 mM sodium bicarbonate, pH 8.6, containing 100 mM sodium chloride and 5% glycerol
UniProt Accession №
NP_001959
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    100 µg

    $502

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    Eukaryotic translation initiation factor 4E (eIF4E) is a component of the eIF4 complex that specifically binds to the methy-7-guanosine cap structure at the 5’ end of eukaryotic mRNA to initiate translation.1,2,3 Nuclear eIF4E promotes export of mRNAs that contain the eIF4E sensitivity element (4E-SE) to the cytosol while cytosolic eIF4E, in a complex with eIF4A and eIF4G1/eIF4G3, mediates recruitment of mRNAs to the ribosome to initiate cap-dependent translation.4,3 eIF14E activity is regulated by eukaryotic translation initiation factor 4E-binding proteins (4E-BPs), which bind eIF4E to prevent eIF4 complex assembly and subsequent translation.3 Overexpression of eIF4E is observed in approximately 30% of human malignancies, including breast, colon, and head and neck carcinomas, and acute myeloid leukemias, particularly M4 and M5 subtypes, as well as prostate cancers.5,6,3 Increased eIF4E expression leads to increased translation of oncogenic mRNAs, such as those encoding VEGF and cyclin D1, and contributes to cancer cell survival and proliferation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroder, K., Hertzog, P.J., Ravasi, T., et al. Interferon-γ: An overview of signals, mechanisms and functions. J. Leukoc. Biol. 75(2), 163-189 (2004).

    2. Haghighat, A., and Sonenberg, N. eIF4G dramatically enhances the binding of eIF4E to the mRNA 5'-cap structure. The Journal of Biological Chemisty 272(35), 21677-21680 (1997).

    3. Piserà, A., Campo, A., and Campo, S. Structure and functions of the translation initiation factor eIF4E and its role in cancer development and treatment. J. Genet. Genomics 45(1), 13-24 (2018).

    4. Marcotrigiano, J., Gingras, A.C., Sonenberg, N., et al. Cocrystal structure of the messenger RNA 5' cap-binding protein (eIF4E) bound to 7-methyl-GDP. Cell 89(6), 951-961 (1997).

    5. Carroll, M., and Borden, K.L. The oncogene eIF4E: Using biochemical insights to target cancer. J. Interferon. Cytokine Res. 33(5), 227-238 (2013).

    6. Yin, X., Kim, R.H., Sun, G., et al. Overexpression of eukaryotic initiation factor 4E is correlated with increased risk for systemic dissemination in node-positive breast cancer patients. J. Am. Coll. Surg. 218(4), 663-671 (2014).