A thymidine nucleoside derivative commonly used to identify proliferating cells
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5-Bromo-2'-deoxyuridine

Item No. 15580

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-bromo-2'-deoxy-uridine
CAS Number
59-14-3
Synonyms
  • BrdU
  • Bromodeoxyuridine
  • Broxuridine
  • NSC 38297
Molecular Formula
C9H11BrN2O5
Formula Weight
307.1
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 20 mg/mlEthanol: 25 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
211, 279 nm
SMILES
OC[C@@H]1[C@@H](O)C[C@H](N2C=C(Br)C(NC2=O)=O)O1
InChi Code
InChI=1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
InChi Key
WOVKYSAHUYNSMH-RRKCRQDMSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    50 mg

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    Product Description

    5-Bromo-2'-deoxyuridine (BrdU) is a thymidine nucleoside derivative that is commonly used to identify proliferating cells.1 In proliferating cells, BrdU is incorporated in place of thymidine during the S phase of the cell cycle during DNA synthesis. Actively proliferating cells can then be detected by denaturing the DNA and allowing specific antibodies to target the incorporated BrdU. Intraperitoneal injection of BrdU is commonly used in combination with neuronal markers to detect adult neurogenesis in animal models.2,3 BrdU is also commonly used as a marker of proliferation of cancer cells in vitro.4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cavanagh, B.L., Walker, T., Norazit, A., et al. Thymidine analogues for tracking DNA synthesis. Molecules 16(9), 7980-7993 (2011).

    2. Wojtowicz, J.M., and Kee, N. BrdU assay for neurogenesis in rodents. Nat. Protoc. 1(3), 1399-1405 (2006).

    3. Kee, N., Sivalingam, S., and Wojtowicz, J.M. The utility of Ki-67 and BrdU as proliferative markers of adult neurogenesis. J. Neurosci. Methods 115(1), 97-105 (2002).

    4. Remvikos, Y., Vielh, P., Padoy, E., et al. Breast cancer proliferation measured on cytological samples: A study by flow cytometry of S-phase fractions and BrdU incorporation. Br. J. Cancer 64(3), 501-507 (1991).