For immunochemical detection of MPO
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Myeloperoxidase Monoclonal Antibody (Clone 5F6)

Item No. 15640

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • MPO
Clone Designation
5F6
Formulation (Request formulation change)
200 µg of affinity-purified monoclonal antibody
Storage Buffer
200 µl PBS, pH 7.2, with 50% glycerol, 0.5 mg/ml BSA, and 0.02% sodium azide
Host
Mouse
Isotype
IgG2b
Applications
IF
Species Reactivity
(+) Human(+) Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea (200 µg)

    $347

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    Product Description

    Myeloperoxidase (MPO) is a heme-containing enzyme and the most abundant protein in polymorphonuclear leukocytes (PMNs).1 It is comprised of two subunits linked by a disulfide bridge with each subunit containing a light and a heavy polypeptide chain. It can oxidize a variety of substrates and catalyzes the formation of highly reactive (pseudo)hypohalous acids and radicals, including hypochlorous acid. MPO is stored in azurophilic granules of PMNs and is released from activated or necrotic PMNs, after which it can bind to and modify acidic serum proteins, as well as recruit additional PMNs. MPO also has roles in PMN apoptosis and antimicrobial defense systems, including neutrophil extracellular traps (NETs).1,2,3 MPO-deficient mice exhibit reduced survival in a polymicrobial sepsis model, increased susceptibility to experimental autoimmune encephalomyelitis (EAE), and increased atherosclerosis in mice also deficient in the LDL receptor and fed an atherogenic diet.1,4,5 Cayman's Myeloperoxidase Monoclonal Antibody (Clone 5F6) was developed by fusing the spleen of a non-immunized NZBWF1 mouse with a mouse myeloma cell line and can be used for immunofluorescence (IF) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Arnhold, J., and Flemmig, J. Human myeloperoxidase in innate and acquired immunity. Arch. Biochem. Biophys. 500(1), 92-106 (2010).

    2. Metzler, K.D., Fuchs, T.A., Nauseef, W.M., et al. Myeloperoxidase is required for neutrophil extracellular trap formation: Implications for innate immunity. Blood 117(3), 953-959 (2011).

    3. Urban, C.F., Ermert, D., Schmid, M., et al. Neutrophil extracellular traps contain calprotectin, a cytosolic protein complex involved in host defense against Candida albicans. PLoS Pathog. 5(10), e1000639 (2009).

    4. Brennan, M., Gaur, A., Pahuja, A., et al. Mice lacking myeloperoxidase are more susceptible to experimental autoimmune encephalomyelitis. J. Neuroimmunol. 112(1-2), 97-105 (2001).

    5. Brennan, M.L., Anderson, M.M., Shih, D.M., et al. Increased atherosclerosis in myeloperoxidase-deficient mice. J. Clin. Invest. 107(4), 419-430 (2001).