A reversible inhibitor of MreB function
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Product Type

Biochemicals
Antibiotics

Research Area

Cell Biology
Cytoskeleton & Motor Proteins

MreB Perturbing Compound A22

Item No. 15870

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
carbamimidothioic acid,(3,4-dichlorophenyl)methyl ester, monohydrochloride
CAS Number
22816-60-0
Molecular Formula
C8H8Cl2N2S • HCl
Formula Weight
271.6
Purity
≥95%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 20 mg/mlDMSO: 20 mg/mlDMSO:PBS (pH 7.2) (1:1): 0.5 mg/mlEthanol: 1 mg/ml
SMILES
ClC1=CC(CSC(N)=N)=CC=C1Cl.Cl
InChi Code
InChI=1S/C8H8Cl2N2S.ClH/c9-6-2-1-5(3-7(6)10)4-13-8(11)12;/h1-3H,4H2,(H3,11,12);1H
InChi Key
VBJNMXMOMSWRDV-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    10 mg

    $81

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    25 mg

    $131

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    50 mg

    $239

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    Product Description

    MreB is an actin-like protein expressed in bacteria.1 It determines the rod shape of cells and has critical roles in cell division, chromosome segregation, and cell polarity.2 MreB perturbing compound A22 is a benzylisothiourea compound that interacts with the ATP binding site of MreB rapidly and reversibly.3 It blocks normal rod shape formation and inhibits chromosome partitioning in E. coli, inhibiting growth (MIC = 3.1 µg/ml).4 It interferes with chromosome segregation in C. crescentus by preventing MreB binding to chromosomes.3 In V. cholerae, A22 prevents the development of normal cell shape and alters nucleoid morphology.5

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Doi, M., Wachi, M., Ishino, F., et al. Determinations of the DNA sequence of the mreB gene and of the gene products of the mre region that function in formation of the rod shape of Escherichia coli cells. J. Bacteriol. 170(10), 4619-4624 (1988).

    2. Tan, Q., Awano, N., and Inouye, M. YeeV is an Escherichia coli toxin that inhibits cell division by targeting the cytoskeleton proteins, FtsZ and MreB. Mol. Microbiol. 79(1), 109-118 (2011).

    3. Gitai, Z., Dye, N.A., Reisenauer, A., et al. MreB actin-mediated segregation of a specific region of a bacterial chromosome. Cell 120(3), 329-341 (2005).

    4. Iwai, N., Nagai, K., and Wachi, M. Novel S-benzylisothiourea compound that induces spherical cells in Escherichia coli probably by acting on a rod-shape-determining protein(s) other than penicillin-binding protein 2. Biosci. Biotechnol. Biochem. 66(12), 2658-2662 (2002).

    5. Srivastava, P. Changes in nucleoid morphology and origin localization upon inhibition or alteration of the actin homolog, MreB, of Vibrio cholerae. J. Bacteriol. 189(20), 7450-7463 (2007).