sPLA₂ (mouse Type V) Polyclonal Antibody

Secretory phospholipase A₂ (sPLA₂) (Type V) is a calcium-dependent phospholipase A₂ superfamily member that is encoded by PLA2G5 in humans.¹ It is composed of a highly conserved calcium-binding loop, a heparin-binding domain, and a C-terminal domain that contains a catalytic histidine-aspartic acid dyad and cationic residues that are essential for cell membrane association. Pla2g5 is constitutively expressed in the heart, lung, and spleen and is induced by proinflammatory stimuli in a variety of immune cells and tissues.² Upon activation, sPLA₂ (Type V) is released into the extracellular space where it acts in a paracrine or autocrine manner to catalyze the hydrolysis of fatty acids at the sn-2 position of glycerophospholipids, liberating the free fatty acid and lysophospholipid, which serve as substrates for the synthesis of bioactive lipid metabolites.¹ Pla2g5^-/- mice have decreased airway and bronchoalveolar lavage fluid (BALF) neutrophil infiltration in a model of acute lung injury induced by LPS.³ Neonatal mice overexpressing Pla2g5 have increased BALF prostaglandin E₂ (PGE₂; Item No. 14010) levels, as well as reduced lung surfactant levels, leading to respiratory failure and death by postnatal day one.⁴ SNPs in PLA2G5 are associated with increased plasma LDL and total cholesterol levels in individuals with type 2 diabetes.⁵ Cayman's sPLA₂ (mouse Type V) Polyclonal Antibody can be used for Western blot applications. The antibody recognizes sPLA₂ (Type V) at 16 kDa from human, mouse, and rat samples.

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1. Murakami, M., Taketomi, Y., Sato, H., et al. Secreted phospholipase A₂ revisited. J. Biochem. 150(3), 233-255 (2011).

2. Sawada, H., Murakami, M., Enomoto, A., et al. Regulation of type V phospholipase A₂ expression and function by proinflammatory stimuli. Eur. J. Biochem. 263(3), 826-833 (1999).

3. Muñoz, N.M., Meliton, A.Y., Meliton, L.N., et al. Secretory group V phospholipase A₂ regulates acute lung injury and neutrophilic inflammation caused by LPS in mice. Am. J. Physiol. Lung Cell. Mol. Physiol. 296(6), L879-L887 (2009).

4. Ohtsuki, M., Taketomi, Y., Arata, S., et al. Transgenic expression of group V, but not group X, secreted phospholipase A₂ in mice leads to neonatal lethality because of lung dysfunction. The Journal of Biological Chemisty 281(47), 36420-36433 (2006).

5. Wootton, P.T.E., Arora, N.L., Drenos, F., et al. Tagging SNP haplotype analysis of the secretory PLA₂-V gene, PLA2G5, shows strong association with LDL and oxLDL levels, suggesting functional distinction from sPLA₂-IIA: Results from the UDACS study. Hum. Mol. Genet. 16(12), 1437-1444 (2007).