For immunochemical detection of the PAF receptor
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PAF Receptor (human) Monoclonal Antibody (11A4, Clone 21)

Item No. 160600

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Platelet-activating Factor Receptor
Immunogen
Synthetic peptide from the internal region of human PAF receptor
Clone Designation
11A4, Clone 21
Formulation (Request formulation change)
100 µg of protein A-purified monoclonal antibody
Storage Buffer
TBS, pH 7.4, when reconstituted in 500 µl of deionized water
Host
Mouse
Isotype
IgG2a
Applications
(+) ELISA, FC, and ICC; (−) WB
Species Reactivity
(+) Human PAF receptor(+) Bovine PAF receptor(+) Porcine PAF receptor
UniProt Accession №
P25105
Origin
Animal/Mouse
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (100 µg)

    $347

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    Product Description

    PAF is a potent phospholipid mediator which exerts diverse biological actions by interaction with a G protein-coupled PAF receptor. The PAF receptor has been cloned from a number of species including human, rat, and guinea pig and is characterized as a 7-transmembrane receptor which induces phosphoinositol turnover through G-protein coupling.1,2,3,4,5 Northern blot analysis reveals that the receptor is expressed in leukocytes, placenta, lung, spleen, small intestine, kidney, liver, and brain.3,4 In leukocyte cell populations the receptor is found on platelets, monocytes, neutrophils, and B-cells, whereas resting T-cells and natural killer cell lines do not express the PAF receptor.6 Human monocytes treated with INF-γ have a 2-6 fold increase in PAF receptor expression compared to untreated cells.7

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Nakamura, M., Honda, Z.i., Izumi, T., et al. Molecular cloning and expression of platelet-activating factor receptor from human leukocytes. The Journal of Biological Chemisty 266(30), 20400-20405 (1991).

    2. Kunz, D., Gerard, N.P., and Gerard, C. The human leukocyte platelet-activating factor receptor. cDNA cloning, cell surface expression, and construction of a novel epitope-bearing analog. The Journal of Biological Chemisty 267, 9101-9106 (1992).

    3. Ye, R.D., Prossnitz, E.R., Zou, A., et al. Characterization of a human cDNA that encodes a functional receptor for platelet activating factor. Biochem. Biophys. Res. Commun. 180, 105-111 (1991).

    4. Bito, H., Honda, Z.i., Nakamura, M., et al. Cloning, expression and tissue distribution of rat platelet-activating-factor-receptor cDNA. Eur. J. Biochem. 227, 211-218 (1994).

    5. Honda, Z.i., Nakamura, M., Miki, I., et al. Cloning by functional expression of platelet-activating factor receptor from guinea-pig lung. Nature 349, 342-346 (1991).

    6. Müller, E., Dagenais, P., Alami, N., et al. Identification and functional characterization of platelet-activating factor receptors in human leukocyte populations using polyclonal anti-peptide antibody. Proc. Natl. Acad. Sci. USA 90, 5818-5822 (1993).

    7. Quellet, S., Müller, E., and Rola-Pleszczynski, M. IFN-γ up-regulates platelet-activating factor receptor gene expression in human monocytes. J. Immunol. 152, 5092-5099 (1994).