For immunochemical detection of the PAF receptor
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PAF Receptor (human) Polyclonal Antibody

Item No. 160602

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Platelet-activating Factor Receptor
Immunogen
Synthetic peptide from the N-terminal region of human PAF receptor
Formulation (Request formulation change)
500 µl of peptide affinity-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Applications
FC and WB
Species Reactivity
(+) Human(+) Green Monkey
UniProt Accession №
P25105
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea (500 µl)

    $347

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    Product Description

    PAF is a potent phospholipid mediator which exerts diverse biological actions by interaction with a G protein-coupled PAF receptor. The PAF receptor has been cloned from a number of species including human, rat, and guinea pig and is characterized as a 7-transmembrane receptor which induces phosphoinositol turnover through G-protein coupling.1,2,3,4,5 Northern blot analysis reveals that the receptor is expressed in leukocytes, placenta, lung, spleen, small intestine, kidney, liver, and brain.3,4 In leukocyte cell populations the receptor is found on platelets, monocytes, neutrophils, and B-cells, whereas resting T-cells and natural killer cell lines do not express the PAF receptor.6 Human monocytes treated with INF-γ have a 2-6 fold increase in PAF receptor expression compared to untreated cells.7 PAF receptor is detected on immunoblot at 48 kDa.8

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Nakamura, M., Honda, Z.i., Izumi, T., et al. Molecular cloning and expression of platelet-activating factor receptor from human leukocytes. The Journal of Biological Chemisty 266(30), 20400-20405 (1991).

    2. Kunz, D., Gerard, N.P., and Gerard, C. The human leukocyte platelet-activating factor receptor. cDNA cloning, cell surface expression, and construction of a novel epitope-bearing analog. The Journal of Biological Chemisty 267, 9101-9106 (1992).

    3. Ye, R.D., Prossnitz, E.R., Zou, A., et al. Characterization of a human cDNA that encodes a functional receptor for platelet activating factor. Biochem. Biophys. Res. Commun. 180, 105-111 (1991).

    4. Bito, H., Honda, Z.i., Nakamura, M., et al. Cloning, expression and tissue distribution of rat platelet-activating-factor-receptor cDNA. Eur. J. Biochem. 227, 211-218 (1994).

    5. Mutoh, H., Bito, H., Minami, M., et al. Two different promoters direct expression of two distinct forms of mRNAs of human platelet-activating factor receptor. FEBS Lett. 322, 129-134 (1993).

    6. Müller, E., Dagenais, P., Alami, N., et al. Identification and functional characterization of platelet-activating factor receptors in human leukocyte populations using polyclonal anti-peptide antibody. Proc. Natl. Acad. Sci. USA 90, 5818-5822 (1993).

    7. Quellet, S., Müller, E., and Rola-Pleszczynski, M. IFN-γ up-regulates platelet-activating factor receptor gene expression in human monocytes. J. Immunol. 152, 5092-5099 (1994).

    8. Marrache, A.M., Gobeil, F., Jr., Bernier, S.G., et al. Proinflammatory gene induction by platelet-activating factor mediated via its cognate nuclear receptor. J. Immunol. 169(11), 6474-6481 (2002).

    Product Citations

    Sutovsky, P., Plummer, W., Baska, K., et al. Relative levels of semen platelet activating factor-receptor (PAFr) and ubiquitin in yearling bulls with high content of semen white blood cells: Implications for breeding soundness evaluation. J. Androl. 28(1), 92-108 (2007).