A novel tool for detecting 15-PGDH
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15-hydroxy Prostaglandin Dehydrogenase Polyclonal Antibody

Item No. 160615

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 15-PGDH
Immunogen
Synthetic peptide from an internal region of human 15-PGDH
Formulation (Request formulation change)
Peptide affinity-purified polyclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Isotype
IgG
Applications
WB
Species Reactivity
(+) Human(+) Baboon(+) Mouse
UniProt Accession №
P15428
Origin
Animal/Rabbit
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    $347

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    Product Description

    NAD+-dependent 15-PGDH catalyzes the oxidation of prostaglandins to 15-keto metabolites, which have greatly reduced biological activity.1 Human NAD+-dependent 15-PGDH is a 266 amino acid protein with a molecular weight of 29 kDa and is expressed in multiple tissues including lung, placenta, and kidney.2 The enzyme belongs to a large family of short-chain alcohol dehydrogenases, which exhibit amino acid identity ranging from 15-30%.3,4 An NADP+-dependent 15-PGDH, which is also part of this enzyme family, exhibits only 20% amino acid identity to the NAD+-dependent enzyme.5 The peptide used as the antigen for preparation of the 15-PGDH antibody is highly conserved across several species (see above), but has only minor homology to other proteins within the short-chain alcohol dehydrogenase family of enzymes (only the alanine and glycine at the N-terminus of the sequence appear to be conserved).3 Cayman’s 15-PGDH Polyclonal Antibody can be used for Western blot applications. The antibody recognizes 15-PGDH at 29 kDa from human, baboon, and mouse samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hansen, H.S. 15-Hydroxyprostaglandin dehydrogenase. A review. Prostaglandins 12(4), 647-679 (1976).

    2. Ensor, C.M., Yang, J.Y., Okita, R.T., et al. Cloning and sequence analysis of the cDNA for human placental NAD+-dependent 15-hydroxyprostaglandin dehydrogenase. The Journal of Biological Chemisty 265, 14888-14891 (1990).

    3. Jörnvall, H., Persson, B., Krook, M., et al. Short-chain dehydrogenases/reductases (SDR). Biochem. 34(18), 6003-6013 (1995).

    4. Krook, M., Marekov, L., and Jörnvall, H. Purification and structural characterization of placental NAD+-linked 15-hydroxyprostaglandin dehydrogenase. The primary structure reveals the enzyme to belong to the short-chain alcohol dehydrogenase family. Biochemistry 29(3), 738-743 (1990).

    5. Wermuth, B. NADP-dependent 15-hydroxyprostaglandin dehydrogenase is homologous to NAD-dependent 15-hydroxyprostaglandin dehydrogenase and other short-chain alcohol dehydrogenases. Prostaglandins 44(1), 5-9 (1992).

    Product Citations

    Bruner-Tran, K.L., Ding, T., Yeoman, K.B., et al. Developmental exposure of mice to dioxin promotes transgenerational testicular inflammation and an increased risk of preterm birth in unexposed mating partners. PLoS One 9(8), e105084 (2014).

    Dai, L., Perera, D.S., King, D.W., et al. Hemokinin-1 stimulates prostaglandin E2 production in human colon through activation of cyclooxygenase-2 and inhibition of 15-hydroxyprostaglandin dehydrogenase. J. Pharmacol. Exp. Ther. 340(1), 27-36 (2012).